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781. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
782. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
783. SEmi-dry transfer blotting - (reply: 1)
784. Whole cell lysis of fibroblasts - (reply: 1)
785. SDS-PAGE: Strange Running - (reply: 8)
786. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
787. Different TBST pH's for WB incubation, why? - (reply: 1)
788. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
789. stability of the stripping buffer - (reply: 2)
790. small proteins have run ahead of tracking dye - (reply: 1)
791. cytoplasmic and nuclear extraction - (reply: 2)
792. Equal Protein Quantity - Loading onto Well (reply: 6)
793. Help, odd pattern - pic included - (reply: 3)
794. Fermentas prestained protein marker issue! - (reply: 7)
795. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
796. Invitrogen NuPAGE Sample buffer - (reply: 1)
797. western exposure one part of band darker - (reply: 6)
798. non-specific bands - (reply: 5)
799. Crazy looking BN-PAGE gel - No, really. (reply: 5)
800. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
801. bands of protein seems like it did not move - (reply: 2)
802. downshifted band - (reply: 4)
803. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
804. Western Troubleshooting Help, please! - (reply: 3)
805. Re-use membrane in Western Blot - (reply: 3)
806. NP40 or Triton as membrane protein detergent - (reply: 2)
807. Dot blot- great, western blot - no signal - (reply: 3)
808. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
809. Problem in transfer high MW protein - (reply: 5)
810. Stacking gel recipe for Acidic Native Gel? - (reply: 1)
782. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
783. SEmi-dry transfer blotting - (reply: 1)
784. Whole cell lysis of fibroblasts - (reply: 1)
785. SDS-PAGE: Strange Running - (reply: 8)
786. Laemmli Buffer Problems - my lanes collapse on themselves (reply: 2)
787. Different TBST pH's for WB incubation, why? - (reply: 1)
788. Ponceau S and Coomasie do not correlate to b actin signal - (reply: 10)
789. stability of the stripping buffer - (reply: 2)
790. small proteins have run ahead of tracking dye - (reply: 1)
791. cytoplasmic and nuclear extraction - (reply: 2)
792. Equal Protein Quantity - Loading onto Well (reply: 6)
793. Help, odd pattern - pic included - (reply: 3)
794. Fermentas prestained protein marker issue! - (reply: 7)
795. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
796. Invitrogen NuPAGE Sample buffer - (reply: 1)
797. western exposure one part of band darker - (reply: 6)
798. non-specific bands - (reply: 5)
799. Crazy looking BN-PAGE gel - No, really. (reply: 5)
800. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
801. bands of protein seems like it did not move - (reply: 2)
802. downshifted band - (reply: 4)
803. no black band but empty stamp of the protein - instead of black bands in xray film, the space is empty with a stamp o (reply: 5)
804. Western Troubleshooting Help, please! - (reply: 3)
805. Re-use membrane in Western Blot - (reply: 3)
806. NP40 or Triton as membrane protein detergent - (reply: 2)
807. Dot blot- great, western blot - no signal - (reply: 3)
808. problem in western blotting, picture attached - spots in xray film but no bands (reply: 4)
809. Problem in transfer high MW protein - (reply: 5)
810. Stacking gel recipe for Acidic Native Gel? - (reply: 1)