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241. about western blot analysis - (reply: 1)
242. Western Blotting loading control for large proteins - (reply: 2)
243. Uneven beta actin - (reply: 2)
244. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
245. NCOR1 western - (reply: 8)
246. Discrepancy between WB and Flow cytometry result - (reply: 5)
247. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
248. Western blotting transfer - (reply: 2)
249. Western Quantification (No BCA???) - (reply: 2)
250. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
251. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
252. Trouble shooting in Far-western blot. - (reply: 1)
253. Transcription/Translation reaction not resolving - (reply: 7)
254. Ponceau red problem: membrane turned red - (reply: 3)
255. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
256. No bands/weird bands on western blot - (reply: 3)
257. Protein ladder not separating - (reply: 6)
258. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
259. Preparing supernatant from cell culture for western blot - (reply: 4)
260. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
261. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
262. Curved bands in Western Blot - (reply: 2)
263. Some bands not coming up, others uneven - (reply: 9)
264. Western blot and Triton X-100 in blocking buffer - (reply: 1)
265. Dot blot translation to WB - (reply: 2)
266. Reference protein give different expression - (reply: 3)
267. Excess betamercaptoethanol during sample preparation - (reply: 4)
268. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
269. Far western blot: probe concentration - (reply: 1)
270. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)
242. Western Blotting loading control for large proteins - (reply: 2)
243. Uneven beta actin - (reply: 2)
244. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
245. NCOR1 western - (reply: 8)
246. Discrepancy between WB and Flow cytometry result - (reply: 5)
247. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
248. Western blotting transfer - (reply: 2)
249. Western Quantification (No BCA???) - (reply: 2)
250. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
251. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
252. Trouble shooting in Far-western blot. - (reply: 1)
253. Transcription/Translation reaction not resolving - (reply: 7)
254. Ponceau red problem: membrane turned red - (reply: 3)
255. Blank spots on WB after semi-dry, NO BUBBLES - (reply: 3)
256. No bands/weird bands on western blot - (reply: 3)
257. Protein ladder not separating - (reply: 6)
258. Western Sample Buffer - DTT vs B-mercaptoethanol - (reply: 5)
259. Preparing supernatant from cell culture for western blot - (reply: 4)
260. A lot of background noise on anti-phosphotyrosine blot? - (reply: 1)
261. Blocking buffer for phospho-(AKT, GSK3b) WB using Odyssey? - (reply: 1)
262. Curved bands in Western Blot - (reply: 2)
263. Some bands not coming up, others uneven - (reply: 9)
264. Western blot and Triton X-100 in blocking buffer - (reply: 1)
265. Dot blot translation to WB - (reply: 2)
266. Reference protein give different expression - (reply: 3)
267. Excess betamercaptoethanol during sample preparation - (reply: 4)
268. Multiple Non-Specific Bands in Western Blot Detected... - (reply: 1)
269. Far western blot: probe concentration - (reply: 1)
270. Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? - (reply: 5)