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1021. Blocking buffer as antibody dilutent? - (reply: 4)
1022. How to prepare colloidal coomassie blue? - ingredients can not dissolve (reply: 4)
1023. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
1024. protein quantification by bradford - (reply: 3)
1025. protein extraction from tumor samples containing matrigel? - (reply: 2)
1026. western blot - western blot (reply: 17)
1027. Amount of protein loaded per well? - (reply: 4)
1028. Casp3 - (reply: 2)
1029. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
1030. taking protein out of membrane - (reply: 1)
1031. prearing gel in advance - (reply: 6)
1032. freezing after reducing and boiling - (reply: 10)
1033. Invitrogen iBlot - (reply: 6)
1034. Even volumes of different samples - (reply: 3)
1035. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
1036. A single band on WB smaller than expected - (reply: 3)
1037. Ponceau staining after primary and secondary? - (reply: 5)
1038. caspase3 detection - help (reply: 5)
1039. Big white spots on PVDF membrane - (reply: 1)
1040. High background.... - (reply: 6)
1041. WB does not correspond to protein array - (reply: 1)
1042. Strange pattern after transfer - (reply: 8)
1043. Quantifying Western blots - (reply: 1)
1044. Complete transfer, can it be done or not? - (reply: 4)
1045. Can add new secondary Ab? - (reply: 1)
1046. Dot blot - (reply: 2)
1047. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
1048. anti-phosphotyrosine blot on 2mo old samples? - (reply: 1)
1049. NATIVE PAGE - (reply: 1)
1050. Looking for new endogenous control for WB - (reply: 3)
1022. How to prepare colloidal coomassie blue? - ingredients can not dissolve (reply: 4)
1023. problem with SDS-PAGE - Sample stuck in the wells of the gel (reply: 5)
1024. protein quantification by bradford - (reply: 3)
1025. protein extraction from tumor samples containing matrigel? - (reply: 2)
1026. western blot - western blot (reply: 17)
1027. Amount of protein loaded per well? - (reply: 4)
1028. Casp3 - (reply: 2)
1029. Anti-FLAG antibody help - Troubleshooting for Sigma anti-FLAG (A8592) (reply: 3)
1030. taking protein out of membrane - (reply: 1)
1031. prearing gel in advance - (reply: 6)
1032. freezing after reducing and boiling - (reply: 10)
1033. Invitrogen iBlot - (reply: 6)
1034. Even volumes of different samples - (reply: 3)
1035. higher MW non specific band - 90-100 kDa band always present, WT or KO (reply: 3)
1036. A single band on WB smaller than expected - (reply: 3)
1037. Ponceau staining after primary and secondary? - (reply: 5)
1038. caspase3 detection - help (reply: 5)
1039. Big white spots on PVDF membrane - (reply: 1)
1040. High background.... - (reply: 6)
1041. WB does not correspond to protein array - (reply: 1)
1042. Strange pattern after transfer - (reply: 8)
1043. Quantifying Western blots - (reply: 1)
1044. Complete transfer, can it be done or not? - (reply: 4)
1045. Can add new secondary Ab? - (reply: 1)
1046. Dot blot - (reply: 2)
1047. SDS PAGE of Bovine Serum albumin - Why more than one band? (reply: 6)
1048. anti-phosphotyrosine blot on 2mo old samples? - (reply: 1)
1049. NATIVE PAGE - (reply: 1)
1050. Looking for new endogenous control for WB - (reply: 3)