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SDS-PAGE-and-Western-Blotting
61.
Rapidly decomposing HRP substrate -
(reply: 2)
62.
protein size in SDS-PAGE -
(reply: 4)
63.
Protein not migrating through gel -
(reply: 9)
64.
Western Sensitivity: ECL versus DyLight 800 Detection -
(reply: 3)
65.
MAb detected on ELISA but no mIgG detected in WB -
(reply: 1)
66.
Extraction of bands from SDS-PAGE -
(reply: 2)
67.
Dumbbell-shaped bands on the WB -
(reply: 3)
68.
visualization of small peptide on tris-ricine sds page gel -
(reply: 2)
69.
Deformed bands in gel -
(reply: 8)
70.
non specific immunoblot -
(reply: 2)
71.
Protein stuck in the origin of resolving gel -
(reply: 12)
72.
detection of small peptide by the SDS-PAGE gel -
(reply: 1)
73.
Help in western blotting GAPDH bands are not even -
(reply: 1)
74.
37kb and 7kb protein in western blot -
(reply: 24)
75.
Dot blot/Western background signal problem -
(reply: 1)
76.
Blocking and Washing membrane -
(reply: 3)
77.
detection of a protein band of protein complex -
(reply: 3)
78.
Cell lysis -
(reply: 1)
79.
SDS-PAGE protein analysis with antibodies alone (not WB) -
(reply: 1)
80.
Western blot detects exogenous protein but not endogenous -
(reply: 2)
81.
White bands in western blotting -
(reply: 4)
82.
Question with the molecular weight of my protein -
(reply: 1)
83.
Possible Protein Aggregation Riddle -
(reply: 3)
84.
How to obtain a control positive for my WB -
(reply: 3)
85.
Mystery of SDS gel, working with the protein GB1 (T2Q mutation) -
(reply: 3)
86.
Antibody not binding to low MW targets -
(reply: 4)
87.
Western blot problem....laddering pattern of white bands on Nitrocellulose membr -
(reply: 5)
88.
Problem with Western Blotting - BCA assay shows protein but no protein bands whe -
(reply: 3)
89.
Question on loss of signal between samples in western blot -
(reply: 4)
90.
IP heavy chains not in Ponceau, but in final blot exposure -
(reply: 1)
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