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SDS-PAGE-and-Western-Blotting
211.
patchy/holey bands after transfer, invasive bubbles? -
(reply: 7)
212.
Western not working -
(reply: 4)
213.
fused bands of beta-actin in western blot -
(reply: 2)
214.
Suggestions - Nice Western Blot Image from Office Scanner? -
(reply: 7)
215.
Undergrad "activated" a NS membrane with 100% MeOH... Any hope? -
(reply: 5)
216.
Problem with GFP detection in western -
(reply: 6)
217.
Sudden Trouble with PVDF Transfer -
(reply: 6)
218.
Equal Loading Confusion -
(reply: 6)
219.
Wrong protein size on western blot -
(reply: 10)
220.
How to know if the signal of western blot is saturated ? -
(reply: 4)
221.
Scotch-Brite versus other foam pad for wet transfer? -
(reply: 3)
222.
5x SDS lysis buffer -
(reply: 5)
223.
Protein measurement of Laemmli samples -
(reply: 2)
224.
SDS Page Problem: Proteins stuck at stacking line -
(reply: 3)
225.
Uneven banding - Western blot troubleshooting -
(reply: 4)
226.
Western Blot with fixed cells -
(reply: 5)
227.
Mix of Milk, BSA, Casein for membrane blocking -
(reply: 3)
228.
Primary antibody -
(reply: 1)
229.
Native-PAGE, help! -
(reply: 3)
230.
Trouble with purified recombinant protein bands -
(reply: 3)
231.
Stain in electrophoresis is going from + to - -
(reply: 3)
232.
Would IP make the protein of interest run slower on SDS-PAGE? -
(reply: 2)
233.
Extraction of Transmembrane Proteins -
(reply: 1)
234.
Western Blot - Quantifying Tumor samples from two different blots -
(reply: 2)
235.
Protocol for detecting soluble, secreted proteins on a Western? -
(reply: 1)
236.
Protein amount to load for publication figure? -
(reply: 4)
237.
Native gel electrophoresis: Can it tell if a protein is forming dimer? -
(reply: 1)
238.
What is different between Native protein and Denatured protein samples. -
(reply: 4)
239.
3 extra bands show up on my western after an IP -
(reply: 2)
240.
IgG heavy chain from IP -
(reply: 8)
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