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331. Addition of sample loading buffer, before or after heating - (reply: 5)
332. question about protein expression normalization. - (reply: 6)
333. Protein bands not migrating - (reply: 3)
334. Help with high background (have tried most trouble shooting) - (reply: 8)
335. Tumor protein precipitation at denaturing step? - (reply: 1)
336. How to prepare blood serum for western blot? - (reply: 3)
337. TNF alpha and IL 1b Western Blot - (reply: 1)
338. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
339. SDS-PAGE trouble with protein concentration - (reply: 2)
340. Acrylamid - (reply: 5)
341. Detecting doublet bands in Western for eGFP control - (reply: 1)
342. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
343. Protein Size 50 kDa higher than expected - (reply: 3)
344. Acid urea gel electrophoresis (AUGE) - (reply: 7)
345. Phospho-protein vs total protein Western blot - (reply: 5)
346. storing the loaded gel before running it - (reply: 7)
347. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
348. Western blot trouble shooting - (reply: 3)
349. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
350. two bands on WB-alternative use of start codons? - (reply: 2)
351. TCA Precipitation to remove albumin - (reply: 6)
352. adjusting pH of 4x stacking buffer - (reply: 3)
353. No WB bands for H2AX at all...! - (reply: 10)
354. any tips for doing Fluorescence Western Blot? - (reply: 2)
355. Unknown silver/grey residue left after transfer. - (reply: 2)
356. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
357. Changes to housekeeping loading controls after drug treatment - (reply: 2)
358. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
359. Multiple bands on blot with one antibody - (reply: 1)
360. sedimentation of cytosolic proteins - (reply: 2)
332. question about protein expression normalization. - (reply: 6)
333. Protein bands not migrating - (reply: 3)
334. Help with high background (have tried most trouble shooting) - (reply: 8)
335. Tumor protein precipitation at denaturing step? - (reply: 1)
336. How to prepare blood serum for western blot? - (reply: 3)
337. TNF alpha and IL 1b Western Blot - (reply: 1)
338. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
339. SDS-PAGE trouble with protein concentration - (reply: 2)
340. Acrylamid - (reply: 5)
341. Detecting doublet bands in Western for eGFP control - (reply: 1)
342. Shelf Life of Tricine-SDS-PAGE gel - (reply: 1)
343. Protein Size 50 kDa higher than expected - (reply: 3)
344. Acid urea gel electrophoresis (AUGE) - (reply: 7)
345. Phospho-protein vs total protein Western blot - (reply: 5)
346. storing the loaded gel before running it - (reply: 7)
347. Help regarding the function of DTT for SDS-PAGE? - (reply: 1)
348. Western blot trouble shooting - (reply: 3)
349. Alcian Blue and Silver Staining for CPS and LPS - (reply: 3)
350. two bands on WB-alternative use of start codons? - (reply: 2)
351. TCA Precipitation to remove albumin - (reply: 6)
352. adjusting pH of 4x stacking buffer - (reply: 3)
353. No WB bands for H2AX at all...! - (reply: 10)
354. any tips for doing Fluorescence Western Blot? - (reply: 2)
355. Unknown silver/grey residue left after transfer. - (reply: 2)
356. How do minimize influence of TissueTek(OCT) in Ripabuffer samples for SDS-Page - (reply: 3)
357. Changes to housekeeping loading controls after drug treatment - (reply: 2)
358. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
359. Multiple bands on blot with one antibody - (reply: 1)
360. sedimentation of cytosolic proteins - (reply: 2)