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New Forum Archives (2009-)
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SDS-PAGE-and-Western-Blotting
811.
dimerization or aggregation of my protein! -
(reply: 3)
812.
basic question in SDS and western blot -
(reply: 1)
813.
Analyzing outer mitochondrial membrane proteins via WB -
(reply: 6)
814.
Transfer problems -
(reply: 1)
815.
Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining
(reply: 15)
816.
Stripping temperature - have no 55C water bath
(reply: 1)
817.
Nonspecificity of anti-flag -
(reply: 2)
818.
Blocking buffer -
(reply: 7)
819.
Detection of phospho-proteins -
(reply: 1)
820.
light chain -
(reply: 5)
821.
ugly merged bands in western blot -
(reply: 3)
822.
Western Blot Data Analysis -
(reply: 2)
823.
Loading Equal protein concentrations - Does it make a difference in simple overexpression checking?
(reply: 4)
824.
Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-(
(reply: 7)
825.
Protein Expression in stationary phase -
(reply: 11)
826.
Protein Smearing -
(reply: 2)
827.
Western blot for muscle -
(reply: 2)
828.
Nupage Bis/Tris Current Voltage problem - Current is so high!
(reply: 12)
829.
Identify the band in western blot -
(reply: 5)
830.
What kind of post-translational modification do you think are these? -
(reply: 2)
831.
Need help with Caspase WB -
(reply: 2)
832.
to keep sds-pa gels -
(reply: 2)
833.
Overexpressed protein not detected - Oh, the frustration...
(reply: 1)
834.
Ponceau staining - Appearence of bands
(reply: 1)
835.
Detecting phosphorylated Erk1/2 -
(reply: 6)
836.
Detecting BDNF with WB... - troubleshooting...
(reply: 2)
837.
western blot using culture media -
(reply: 4)
838.
Re-inserting marker into scanned WB -
(reply: 1)
839.
pre stained marker issues -
(reply: 2)
840.
Functions of BSA and Tween 20 in western blotting -
(reply: 2)
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