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Vertical Vs horizontal electrophoresis - (Jun/10/2012 )

HI

on comparison of vertical and horizontal electrophoresis what are ones advantages and disadvantages ???

-rick112-

For what samples?

-bob1-

in general, what is the advantage of one over the other ???

-rick112-

It depends on what you are trying to do - (assuming you mean acrylamide gels here too) vertical generally = thin gels, good resolution, but smaller sample volumes. horizontally= thick gel, poorer resolution but much bigger samples, easier to access for cutting out bands/band elution.

-bob1-