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631. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
632. RIPA Buffer turns Bradford into Blue - (reply: 2)
633. freezing antibody lyophilised - (reply: 2)
634. Nitrocellulose membrane storage after transfer - (reply: 6)
635. western without tween - (reply: 3)
636. Bubbles on the marker after transfer - (reply: 2)
637. Missing patches on immunoblot - please help! - (reply: 7)
638. stupid question - (reply: 8)
639. GAPDH merging lanes - (reply: 1)
640. Band at same position using different primary antibodies - (reply: 3)
641. Western for very small protein - (reply: 3)
642. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
643. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
644. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
645. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
646. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
647. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
648. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
649. What cause "downshift" in SDS-PAGE? - (reply: 5)
650. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
651. Blue native PAGE problems - (reply: 1)
652. running problems SDS-PAGE gel (zymography) - (reply: 4)
653. Western unresolved lanes - (reply: 2)
654. Doing WB from scratch for the first time. Need some info. - (reply: 2)
655. Western blot bovine collagen typ I problem - (reply: 3)
656. no band, extremely clean blot - (reply: 1)
657. Acrylamide stock - (reply: 2)
658. low signal in WB - (reply: 3)
659. standard internal marker for quantification - (reply: 3)
660. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)
632. RIPA Buffer turns Bradford into Blue - (reply: 2)
633. freezing antibody lyophilised - (reply: 2)
634. Nitrocellulose membrane storage after transfer - (reply: 6)
635. western without tween - (reply: 3)
636. Bubbles on the marker after transfer - (reply: 2)
637. Missing patches on immunoblot - please help! - (reply: 7)
638. stupid question - (reply: 8)
639. GAPDH merging lanes - (reply: 1)
640. Band at same position using different primary antibodies - (reply: 3)
641. Western for very small protein - (reply: 3)
642. sds page - hot hot hot - buffer too hot - gel burnt (reply: 6)
643. COMPARING SDS-PAGE SYSTEMS - Need to buy new system - Member's comments on available systems (reply: 7)
644. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
645. bio-rad mini trans-blot issue - newer tetra tank decreases transfer efficiency (reply: 1)
646. Western Blot Secondary Antibody Diluent - basic question (reply: 3)
647. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
648. Re-use PVDF membran without stripping - may I re-block? (reply: 5)
649. What cause "downshift" in SDS-PAGE? - (reply: 5)
650. Weak Zymography Signals - Mitigating a weak signal for better bands (reply: 1)
651. Blue native PAGE problems - (reply: 1)
652. running problems SDS-PAGE gel (zymography) - (reply: 4)
653. Western unresolved lanes - (reply: 2)
654. Doing WB from scratch for the first time. Need some info. - (reply: 2)
655. Western blot bovine collagen typ I problem - (reply: 3)
656. no band, extremely clean blot - (reply: 1)
657. Acrylamide stock - (reply: 2)
658. low signal in WB - (reply: 3)
659. standard internal marker for quantification - (reply: 3)
660. Antibody too much? - Signal not consistent, and fades quickly (reply: 5)