|
721. Expression by ICC but not by Western-why? - (reply: 3)
722. Re-Imaging without Stripping - (reply: 4)
723. MES buffer turned yellow - (reply: 5)
724. western blot woes - (reply: 2)
725. Size larger than expected - totally boggled - (reply: 2)
726. Ponceau Red Staining Problem - (reply: 2)
727. SDS-PAGE Second interface - (reply: 3)
728. biorad's 30% bis/acrylamide storage - (reply: 2)
729. protein concentration per well - (reply: 6)
730. dot blot - Problem (reply: 1)
731. Clear native PAGE - (reply: 1)
732. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
733. Weak western signal for help - (reply: 13)
734. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
735. Western not working anymore - (reply: 15)
736. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
737. BN-PAGE possible with Tris-glycine - (reply: 1)
738. Western few questions - (reply: 1)
739. thiourea in 2D page - What's it for? (reply: 1)
740. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
741. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
742. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
743. Antibody works in immunofluorescence, but not in WB - (reply: 4)
744. B-actin as western blot -control - (reply: 4)
745. horrific western blots , please help - (reply: 3)
746. Trouble with actin, HA and CCND1 :(( - (reply: 1)
747. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
748. Lower MW bands than expected. - (reply: 1)
749. Question about reducing agent (DTT) - (reply: 1)
750. Collagen II western blotting - (reply: 1)
722. Re-Imaging without Stripping - (reply: 4)
723. MES buffer turned yellow - (reply: 5)
724. western blot woes - (reply: 2)
725. Size larger than expected - totally boggled - (reply: 2)
726. Ponceau Red Staining Problem - (reply: 2)
727. SDS-PAGE Second interface - (reply: 3)
728. biorad's 30% bis/acrylamide storage - (reply: 2)
729. protein concentration per well - (reply: 6)
730. dot blot - Problem (reply: 1)
731. Clear native PAGE - (reply: 1)
732. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
733. Weak western signal for help - (reply: 13)
734. No bands in cytosolic extraction, nice bands in nuclear extraction--see pics - (reply: 1)
735. Western not working anymore - (reply: 15)
736. Problem regarding Anti-Na+ Channel Ab - (reply: 3)
737. BN-PAGE possible with Tris-glycine - (reply: 1)
738. Western few questions - (reply: 1)
739. thiourea in 2D page - What's it for? (reply: 1)
740. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
741. Sample reduction/alkylation in urea before SDS-PAGE - (reply: 1)
742. Why should we NOT adjust the pH of running buffer? - Would adjusting the pH cause problems during transfer/downstream steps (reply: 5)
743. Antibody works in immunofluorescence, but not in WB - (reply: 4)
744. B-actin as western blot -control - (reply: 4)
745. horrific western blots , please help - (reply: 3)
746. Trouble with actin, HA and CCND1 :(( - (reply: 1)
747. The end of the stacking gel? - EZ-Run, PRIME gel, NEXT GEL, etc. (reply: 1)
748. Lower MW bands than expected. - (reply: 1)
749. Question about reducing agent (DTT) - (reply: 1)
750. Collagen II western blotting - (reply: 1)