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SDS-PAGE-and-Western-Blotting
511.
Bands from the first detection appear at the second detection but with a differe -
(reply: 1)
512.
gH2A.X blotting problem -
(reply: 1)
513.
Re-using membranes with ghost bands -
(reply: 2)
514.
large protein -
(reply: 3)
515.
Low Molecular Weight Housekeeping Gene for Loading Control? -
(reply: 2)
516.
problem with loading the protein on to SDS-Gel -
(reply: 1)
517.
Western blot not showing protein -
(reply: 2)
518.
normalisation of western blot from kodak film -
(reply: 1)
519.
antibody in 40% glycerol -
(reply: 6)
520.
longer running of proteins in SDS PAGE! -
(reply: 2)
521.
dirty back ground after exposure -
(reply: 2)
522.
Tenfold PBS in extraction -
(reply: 3)
523.
Separating close bands in Western Blot -
(reply: 3)
524.
Western blot on intestine samples -
(reply: 2)
525.
dot blot -
(reply: 4)
526.
ECL Prime Streamlining and Troubleshootin -
(reply: 1)
527.
Samples leaking out of wells in gel -
(reply: 2)
528.
phosphate + freeze thaw vs RIPA buffer for cell lysis -
(reply: 2)
529.
How much cytoplasmic and nuclear extracts load in a WB? -
(reply: 2)
530.
How to extract total protein from gram negative bacteria for SDS page and BCA an -
(reply: 2)
531.
Strange E.coli growth -
(reply: 3)
532.
Problem with Immunoprecipitated samples -
(reply: 2)
533.
problems in transfer -
(reply: 3)
534.
Phospho-JNK -
(reply: 9)
535.
Bradford reagent for staining gels? -
(reply: 1)
536.
developing film manually -
(reply: 19)
537.
non specific bands -
(reply: 7)
538.
BSA vs Milk -
(reply: 2)
539.
Western Blot no target bands, show Non-specific bands -
(reply: 5)
540.
how critical is the pH of tbst? -
(reply: 2)
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