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SDS-PAGE-and-Western-Blotting
391.
Use of wrong TBS concentration -
(reply: 1)
392.
Transfer time optimisation - Overnight low voltage vs Few hours high voltage -
(reply: 1)
393.
SDS-PAGE staining for digestion assay -
(reply: 1)
394.
Can I improve my primary antibody? -
(reply: 2)
395.
got band in control positive but not in sample... -
(reply: 2)
396.
turboGFP Ab for EGFP detection in WB, posible? -
(reply: 1)
397.
Problems with IPs -
(reply: 2)
398.
Band is at the wrong size, but same for all antibodies -
(reply: 6)
399.
Problems with western blot detection -
(reply: 1)
400.
Methods of concentrating total protein extracts -
(reply: 2)
401.
Using serum as the sample in Western blot -
(reply: 3)
402.
Puzzeling western blot results, missing band -
(reply: 3)
403.
Secondary AB blocking -
(reply: 1)
404.
western blood immunodetection Ab -
(reply: 1)
405.
Excess bromophenol blue in 2-D rehydration buffer -
(reply: 1)
406.
Best gel percentage to separate 24 and 36 kDa proteins -
(reply: 3)
407.
What does percentage mean when dealing with stacking and running gel for SDS pag -
(reply: 6)
408.
What percentage should I use for a stacking gel? -
(reply: 1)
409.
DMSO -
(reply: 1)
410.
several questions on running SDS-PAGE -
(reply: 1)
411.
Voltage issues during transfer -
(reply: 5)
412.
problems with housekeeping antibodies when re-probing membranes -
(reply: 3)
413.
What percentage gel should I make? -
(reply: 1)
414.
Western Blot problems-vertical streaking -
(reply: 3)
415.
Protein extraction from dead cells -
(reply: 1)
416.
Signal stuck on top of seperating gel -
(reply: 1)
417.
SDS-PAGE gel and running buffer -
(reply: 3)
418.
The same strong bands with different primary antibody -
(reply: 1)
419.
Trouble Western Blotting a 360kDa Protein -
(reply: 5)
420.
pERK and pAKT in MDA-MB-231 cells by western blot! -
(reply: 1)
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