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481. Protection Lanes? - (reply: 4)
482. Tie-2 westerns high background black lanes - (reply: 13)
483. BSA concentration for blocking - (reply: 5)
484. Identically loaded western bands loose intensity from left to right - (reply: 3)
485. eIF4G western blot - (reply: 3)
486. smear bands in western blot - (reply: 12)
487. Huge background with HT22 cells phophoERK antibody - (reply: 3)
488. How to quantify the protein amount for serum samples ? - (reply: 2)
489. Huge background with HT22 cells phophoERK antibody - (reply: 1)
490. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
491. horizontal smear? - (reply: 2)
492. BSA versus milk in blocking and antibody incubation - (reply: 5)
493. Western blots are 'semi' quantitative? - (reply: 12)
494. bands fuse together - (reply: 1)
495. Phospho-protein detection - (reply: 2)
496. Help on using different antibodies in the same membrane - (reply: 8)
497. weird query for Western Blotting - (reply: 2)
498. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
499. Quick/One-step/automated Western Blot - (reply: 8)
500. Amount of antibody used in Western Blot - (reply: 1)
501. WB Transfer: Voltage vs. Current - (reply: 7)
502. Large protein blot ~400kDa - (reply: 3)
503. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
504. Stripping buffer for nitrocellulose membrane - (reply: 1)
505. PAGE for Separation of DNA - (reply: 3)
506. Western Blotting of Proteins less than 10 kDa - (reply: 1)
507. dot blot - (reply: 7)
508. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
509. Internal Standard for western blot - (reply: 1)
510. Sox-2 antibody detection using Western - (reply: 10)
482. Tie-2 westerns high background black lanes - (reply: 13)
483. BSA concentration for blocking - (reply: 5)
484. Identically loaded western bands loose intensity from left to right - (reply: 3)
485. eIF4G western blot - (reply: 3)
486. smear bands in western blot - (reply: 12)
487. Huge background with HT22 cells phophoERK antibody - (reply: 3)
488. How to quantify the protein amount for serum samples ? - (reply: 2)
489. Huge background with HT22 cells phophoERK antibody - (reply: 1)
490. Loading buffer with beta mercaptoethanol = toxic - (reply: 4)
491. horizontal smear? - (reply: 2)
492. BSA versus milk in blocking and antibody incubation - (reply: 5)
493. Western blots are 'semi' quantitative? - (reply: 12)
494. bands fuse together - (reply: 1)
495. Phospho-protein detection - (reply: 2)
496. Help on using different antibodies in the same membrane - (reply: 8)
497. weird query for Western Blotting - (reply: 2)
498. Reusing SDS-PAGE running and transfer buffers? - (reply: 1)
499. Quick/One-step/automated Western Blot - (reply: 8)
500. Amount of antibody used in Western Blot - (reply: 1)
501. WB Transfer: Voltage vs. Current - (reply: 7)
502. Large protein blot ~400kDa - (reply: 3)
503. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
504. Stripping buffer for nitrocellulose membrane - (reply: 1)
505. PAGE for Separation of DNA - (reply: 3)
506. Western Blotting of Proteins less than 10 kDa - (reply: 1)
507. dot blot - (reply: 7)
508. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
509. Internal Standard for western blot - (reply: 1)
510. Sox-2 antibody detection using Western - (reply: 10)