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31. gelatin zymogram - (reply: 1)
32. No or Super Strong Signal - (reply: 4)
33. Blotchy Blots - (reply: 2)
34. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
35. Dirty blots/high background - (reply: 5)
36. Heating to denature and reduce protein: same temperature? - (reply: 1)
37. Neurotoxicity of acrylamide - (reply: 2)
38. 2 bands very close in the expected place - (reply: 2)
39. Pressing Too Hard on Transfer Sandwich - (reply: 3)
40. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
41. SDS-PAGE High MW bands dissapear! - (reply: 7)
42. Non-specific binding of protein in western blot - (reply: 3)
43. SDS-PAGE vertical lane streaking - (reply: 1)
44. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
45. Storing membrane after antibody incubation - (reply: 2)
46. layer in between resolving and stacking gel - (reply: 1)
47. Lane spillover (?) on Western Blot - (reply: 3)
48. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
49. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
50. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
51. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
52. No band detected in WB - (reply: 4)
53. Concentration of Sample Buffer to boil protein samples - (reply: 2)
54. Western Blot diffuse bands - (reply: 3)
55. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
56. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
57. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
58. Some bands transferred, some not..? Why??? - (reply: 1)
59. Digital imager questions on correction - (reply: 4)
60. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
32. No or Super Strong Signal - (reply: 4)
33. Blotchy Blots - (reply: 2)
34. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
35. Dirty blots/high background - (reply: 5)
36. Heating to denature and reduce protein: same temperature? - (reply: 1)
37. Neurotoxicity of acrylamide - (reply: 2)
38. 2 bands very close in the expected place - (reply: 2)
39. Pressing Too Hard on Transfer Sandwich - (reply: 3)
40. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
41. SDS-PAGE High MW bands dissapear! - (reply: 7)
42. Non-specific binding of protein in western blot - (reply: 3)
43. SDS-PAGE vertical lane streaking - (reply: 1)
44. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
45. Storing membrane after antibody incubation - (reply: 2)
46. layer in between resolving and stacking gel - (reply: 1)
47. Lane spillover (?) on Western Blot - (reply: 3)
48. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
49. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
50. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
51. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
52. No band detected in WB - (reply: 4)
53. Concentration of Sample Buffer to boil protein samples - (reply: 2)
54. Western Blot diffuse bands - (reply: 3)
55. Finding suitable Acrylamide/Bis-acrylamide ratio - (reply: 2)
56. Bubbles in western blot gel "AFTER" running gel - (reply: 2)
57. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
58. Some bands transferred, some not..? Why??? - (reply: 1)
59. Digital imager questions on correction - (reply: 4)
60. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)