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121. Problem with thawed samples - (reply: 3)
122. Problems with histones in SDS-PAGE - (reply: 1)
123. Stripping problem - (reply: 5)
124. Problems of beta-actin - (reply: 1)
125. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
126. Problems of western signal - (reply: 5)
127. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
128. 2x laemmli buffer - (reply: 4)
129. Relative humidity in the electrophoresis lab - (reply: 3)
130. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
131. how can i explain the different pattern from my labmate's one - (reply: 8)
132. Ladder and proteins do not migrate into stacking gel - (reply: 5)
133. Western Blot: Unequal Sample Migration - (reply: 4)
134. Western blot with smeared bands - (reply: 3)
135. SDS PAGE sample preparation question, and discussion? - (reply: 3)
136. too much heating LDS-sample buffer - (reply: 3)
137. problem of cell lysate - (reply: 3)
138. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
139. Blocking agent in western blotting of histone proteins - (reply: 1)
140. Best western loading control for comparing different cell lines - (reply: 2)
141. western blotting running problem? - (reply: 2)
142. Keeping apoptotic/detached cells for WB? - (reply: 3)
143. Advice with WB please - (reply: 3)
144. Western Blot Wet Transfer Conditions - (reply: 10)
145. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
146. Western Blot and Inmunofluorescence contradictions - (reply: 12)
147. Nanog double band in WB - (reply: 1)
148. My beta actin bands are changing - (reply: 6)
149. ECL Substrate Choices - (reply: 2)
150. Why does my gel have streaks instead of bands? - (reply: 2)
122. Problems with histones in SDS-PAGE - (reply: 1)
123. Stripping problem - (reply: 5)
124. Problems of beta-actin - (reply: 1)
125. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
126. Problems of western signal - (reply: 5)
127. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
128. 2x laemmli buffer - (reply: 4)
129. Relative humidity in the electrophoresis lab - (reply: 3)
130. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
131. how can i explain the different pattern from my labmate's one - (reply: 8)
132. Ladder and proteins do not migrate into stacking gel - (reply: 5)
133. Western Blot: Unequal Sample Migration - (reply: 4)
134. Western blot with smeared bands - (reply: 3)
135. SDS PAGE sample preparation question, and discussion? - (reply: 3)
136. too much heating LDS-sample buffer - (reply: 3)
137. problem of cell lysate - (reply: 3)
138. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
139. Blocking agent in western blotting of histone proteins - (reply: 1)
140. Best western loading control for comparing different cell lines - (reply: 2)
141. western blotting running problem? - (reply: 2)
142. Keeping apoptotic/detached cells for WB? - (reply: 3)
143. Advice with WB please - (reply: 3)
144. Western Blot Wet Transfer Conditions - (reply: 10)
145. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
146. Western Blot and Inmunofluorescence contradictions - (reply: 12)
147. Nanog double band in WB - (reply: 1)
148. My beta actin bands are changing - (reply: 6)
149. ECL Substrate Choices - (reply: 2)
150. Why does my gel have streaks instead of bands? - (reply: 2)