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301. Peculiar background problem with MPM-2 antibodies - (reply: 2)
302. Stripping the membrane for LiCor - (reply: 1)
303. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
304. Lysis buffer - (reply: 2)
305. SDS Page and WB: Wrong size bands - (reply: 2)
306. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
307. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
308. How to avoid gel breakage after electrophoresis - (reply: 5)
309. What is an Input in SDS-PAGE? - (reply: 1)
310. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
311. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
312. Good loading control for native gels? - (reply: 1)
313. Please help with odd bands and smears on Western blot - (reply: 4)
314. How to load equal amount of protein without using spectramax - (reply: 4)
315. Western blot membrane imaging - (reply: 7)
316. Detection of T7 RNA polymerase by WB - (reply: 6)
317. Shift in the position of band onto the western blot - (reply: 3)
318. Ghost and inverse bands and high background. Help! - (reply: 3)
319. No resolving of protein/bands below 50kDa :-s - (reply: 5)
320. Problems with wet transfer blot - (reply: 2)
321. 5% BSA blocking buffer - (reply: 3)
322. Storage of Membrane before addition of primary antibody - (reply: 3)
323. "Mickey Mouse" bands - (reply: 5)
324. SDS-PAGE : when the transfer stops - (reply: 1)
325. confusing Western results - (reply: 5)
326. aditional band on WB - (reply: 1)
327. Transferring >150 kDA proteins conditions - (reply: 3)
328. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
329. TAU gel electrophoresis of histones - (reply: 4)
330. Bands dark on sides and light in middle - (reply: 7)
302. Stripping the membrane for LiCor - (reply: 1)
303. 17 kDa ideal transfer voltage - PVDF - (reply: 4)
304. Lysis buffer - (reply: 2)
305. SDS Page and WB: Wrong size bands - (reply: 2)
306. the intensity ratio between light chain and heavy chain on SDS-PAGE - (reply: 3)
307. How to use miniblotter to perform multiple antibody detection on the same membra - (reply: 3)
308. How to avoid gel breakage after electrophoresis - (reply: 5)
309. What is an Input in SDS-PAGE? - (reply: 1)
310. HELP!! Anti-GFP antibody resuspended in 20% SDS!!!! - (reply: 2)
311. Laemmli buffer a bit green or yellow after boiling!!?!? - (reply: 3)
312. Good loading control for native gels? - (reply: 1)
313. Please help with odd bands and smears on Western blot - (reply: 4)
314. How to load equal amount of protein without using spectramax - (reply: 4)
315. Western blot membrane imaging - (reply: 7)
316. Detection of T7 RNA polymerase by WB - (reply: 6)
317. Shift in the position of band onto the western blot - (reply: 3)
318. Ghost and inverse bands and high background. Help! - (reply: 3)
319. No resolving of protein/bands below 50kDa :-s - (reply: 5)
320. Problems with wet transfer blot - (reply: 2)
321. 5% BSA blocking buffer - (reply: 3)
322. Storage of Membrane before addition of primary antibody - (reply: 3)
323. "Mickey Mouse" bands - (reply: 5)
324. SDS-PAGE : when the transfer stops - (reply: 1)
325. confusing Western results - (reply: 5)
326. aditional band on WB - (reply: 1)
327. Transferring >150 kDA proteins conditions - (reply: 3)
328. Does anyone have a reoccuring problem executing Gel electrophoresis? - (reply: 1)
329. TAU gel electrophoresis of histones - (reply: 4)
330. Bands dark on sides and light in middle - (reply: 7)