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SDS-PAGE-and-Western-Blotting
901.
cytochrome C Western Blot -
(reply: 7)
902.
Protein size and gel percentage -
(reply: 7)
903.
Bands visible in membrane but not in the film - ??
(reply: 10)
904.
non desired brigther bands -
(reply: 7)
905.
Dimer or monomer -
(reply: 11)
906.
Protein precipitation by TCA acid + acetone -
(reply: 4)
907.
Non Reducing SDS protein ladder?? -
(reply: 4)
908.
Protein Free Blocking Buffers -
(reply: 1)
909.
Does biotinylated proteins will migrate diferently in an SDS than the actual pro -
(reply: 7)
910.
problem: doublets and triplets of bands occur in SDS-PAGE -
(reply: 5)
911.
SDS-Page issue -
(reply: 5)
912.
Fluorescent visualization of Western blot -
(reply: 2)
913.
MEthanol in NC membrane -
(reply: 7)
914.
Pre stained markers -
(reply: 4)
915.
Laminin 5 antibody -
(reply: 1)
916.
Molecular weight marker - got different pattern!
(reply: 13)
917.
SDS ladder - which type works better?
(reply: 3)
918.
Membrane dried after secondary antibody - can I still develop color?
(reply: 6)
919.
the sensitive amount of protein for western blotting -
(reply: 3)
920.
Validate SDS-Page (Collagen Typ I) - Looking for help about the point: specificity
(reply: 3)
921.
Mouse Fibroblasts - need to extract nuclar protein
(reply: 4)
922.
How to determine final amount of 20 ng protein per WB well -
(reply: 8)
923.
Milk in TBS or TBS-T - TBS with milk becomes clear wenn incubated o/n
(reply: 4)
924.
nonreducing antibody in reducing condition -
(reply: 1)
925.
High Molecular weight protein separation -
(reply: 2)
926.
Secreted protein for loading control -
(reply: 2)
927.
Only borders stained in Silver!!! -
(reply: 7)
928.
Problems in runnging Native gel - Problems in runnging Native gel
(reply: 4)
929.
How Best to Clean the Sonicator Tip Between Samples? -
(reply: 6)
930.
bromophenol blue -
(reply: 10)
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