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SDS-PAGE and Western Blotting
1081.
Phospatase inhibitors needed for LDS Buffer? -
(reply: 3)
1082.
Protein much larger on Western blot than predicted -
(reply: 12)
1083.
how to deal with PVDF filter after WB -
(reply: 5)
1084.
Proteins not running till the end of the gel -
(reply: 2)
1085.
Homemade ECL and Amersham HRP secondary -
(reply: 1)
1086.
Recombinant Protein migration - Doubts on protein migration
(reply: 5)
1087.
How does the protein fixation on SDS gel work? -
(reply: 3)
1088.
Guanidinium thiocyanate and SDS-PAGE -
(reply: 3)
1089.
lysate buffer - function of each components.
(reply: 6)
1090.
lysing cells in LSB -
(reply: 2)
1091.
Problem with running SDS page gel -
(reply: 6)
1092.
Protein extraction from cell culture media -
(reply: 9)
1093.
Problem with the SDS-PAGE sample prepaeration?!! -
(reply: 6)
1094.
Problems with SDS gel - Boiling samples, autoclaved runnung buffer, 4 layers
(reply: 6)
1095.
Homemade ECL imaging? - Can I make a digital blot capture system for my home?
(reply: 7)
1096.
Multiple antibody blotting - western blot -
(reply: 16)
1097.
FISH BLOCK -
(reply: 3)
1098.
native PAGE? - procedures and tips?
(reply: 2)
1099.
Is immunoblotting possible after the gel has been silver stained -
(reply: 2)
1100.
Could this liver homogenate be used for Western Blot? -
(reply: 2)
1101.
protein disapeared from gel! -
(reply: 11)
1102.
Western Blotting- A beginner Guide - Abcam
(reply: 3)
1103.
PVDF vs. Nitrocellulose for Western blot -
(reply: 20)
1104.
Loading control for nuclear extracts -
(reply: 6)
1105.
protein band size determination - any software available?
(reply: 2)
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