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SDS-PAGE-and-Western-Blotting
541.
Fc fusion protein smearing on SDS PAGE -
(reply: 2)
542.
what do you recommend for Semi-Dry unit -
(reply: 2)
543.
Bands disappear after longer exposure -
(reply: 4)
544.
Housekeeper for Plasma samples in Western Blotting -
(reply: 3)
545.
Transfer not occurring on right hand side of membrane correctly -
(reply: 3)
546.
Antibody Optimization -
(reply: 2)
547.
Protein agregation in WB samples -
(reply: 3)
548.
Same Non-specific bands show up for different Abs!! -
(reply: 7)
549.
what caused bubble band of my WB? -
(reply: 4)
550.
blotting detection -
(reply: 3)
551.
Max amount of protein -
(reply: 4)
552.
Areas of membrane bleached -
(reply: 1)
553.
asking suggestions for western blot -
(reply: 3)
554.
Can i apply primary antibody for two nights instead of one? -
(reply: 3)
555.
Strange bands and large smear in western blot -
(reply: 4)
556.
Freezing proteins at -80 -
(reply: 1)
557.
Tris glycine SDS buffer prep pH error -
(reply: 2)
558.
GAPDH - Non-specific bands with GAPDH monoclonal antibody
(reply: 1)
559.
Can Current affect to the samples in the edge/periphery of the gel? -
(reply: 1)
560.
Use of colloidal coomassie stained to western blotting?? -
(reply: 5)
561.
problems with negative control -
(reply: 2)
562.
urgent-W.B visualisation on kodak film -
(reply: 2)
563.
NP 40 lysis buffer -
(reply: 1)
564.
Dot-blot with immobilized ABs on membrane for protein detection -
(reply: 8)
565.
Dark western blot film -
(reply: 12)
566.
how to get red of high secondary signal -
(reply: 4)
567.
strange and branch like bands on SDS-PAGE - troubleshooting
(reply: 7)
568.
Detecting phosphoproteins - different results when samples rerun
(reply: 1)
569.
Is loading buffer ok? -
(reply: 1)
570.
Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels?
(reply: 1)
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