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New Forum Archives (2009-)
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SDS-PAGE-and-Western-Blotting
991.
Ponceau Staining Question -
(reply: 1)
992.
Beginner Question: Membrane position during Blocking and washing? -
(reply: 1)
993.
bubbles between gel and glass plate -
(reply: 9)
994.
Want to detect oligomers - Need advice on native PAGE
(reply: 2)
995.
2D SDS-PAGE - wave spots problem - problem with pattern distorsion - "wave spots"
(reply: 3)
996.
Primary antibody incubation length -
(reply: 3)
997.
How to extract protein from SDS-PAGE gel? -
(reply: 7)
998.
colorimetry vs chemiluminescence -
(reply: 1)
999.
antribody gone old? -
(reply: 11)
1000.
Yellow Laemmli sample buffer -
(reply: 6)
1001.
Antibody Characterization - KO vs. WT or Heterologous overexpression
(reply: 8)
1002.
does 1% SDS prevents proteases? -
(reply: 8)
1003.
Guanidinium Chloride -
(reply: 11)
1004.
many bands of smaller size than expected -
(reply: 3)
1005.
how to solve fungus problem on SDS PAGE gel -
(reply: 4)
1006.
Unusually viscous samples - Protein Sample Preparation
(reply: 2)
1007.
SDS page problems -
(reply: 1)
1008.
SDS-PAGE irregular and difuse bands - what's your opinion?
(reply: 8)
1009.
SDS-PAGE Cell lysis problems -
(reply: 3)
1010.
Using Photoshop etc on Western bot images -
(reply: 8)
1011.
Ghost bands in western blot - Western blotting problems
(reply: 9)
1012.
Why immunoblotting of actin coming at higher Molec. Weight -
(reply: 1)
1013.
x-ray films vs direct imaging - Preferred method for western development
(reply: 17)
1014.
proteins stuck at interface between stack and separating gel -
(reply: 2)
1015.
Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins
(reply: 16)
1016.
Lost my proteins ! -
(reply: 3)
1017.
beginner's Questions on Western Blotting -
(reply: 4)
1018.
lost signal -
(reply: 4)
1019.
Using NaN3 to quench HRP conjugated secondary Ab -
(reply: 1)
1020.
White speckles on western blots - Why?? :(
(reply: 3)
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