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SDS-PAGE-and-Western-Blotting
451.
Problems with protein estimation and western blots -
(reply: 1)
452.
Loading controls -
(reply: 1)
453.
Quantity of protein to charge -
(reply: 1)
454.
Birdlike bands/lanes of SDS-Page and WB -
(reply: 2)
455.
Tris-Hcl becomes cloudy. -
(reply: 3)
456.
Can FLAG IP, but cannot detect with Western -
(reply: 4)
457.
my protein bands and protein marker becomes abnormally clearer sometimes -
(reply: 3)
458.
Redoing only the Secondary Antibody staining on a Western -
(reply: 1)
459.
Native PAGE, interaction of proteins, validity -
(reply: 4)
460.
Smeared transfer on nitrocellulose membrane -
(reply: 2)
461.
blocking and primary antibody in different buffers? -
(reply: 1)
462.
Same non specific band for different target proteins -
(reply: 1)
463.
Types of 96-well plates used for BCA protein quantification -
(reply: 1)
464.
Dot blot halo -
(reply: 4)
465.
help: i accidentally use transfer buffer as running buffer, what will happen??? -
(reply: 4)
466.
Western blot and isoelectric point -
(reply: 1)
467.
Western Blot Developing Cassette -
(reply: 1)
468.
Pull down assay and Co- IP experiment -
(reply: 6)
469.
Trouble detecting Histaq -
(reply: 4)
470.
Western gels running super slow! -
(reply: 3)
471.
a band that suppose to be 90KD shows up at 70KD!! -
(reply: 1)
472.
Extra band on western blot of Insulin Recetor beta subunit (C-19) antibody -
(reply: 1)
473.
WB parametric or non-parametric -
(reply: 6)
474.
Trouble with the GFP signal -
(reply: 1)
475.
Intern Needing Help -
(reply: 3)
476.
SDS-Electrophoresis, Dye color change -
(reply: 1)
477.
Reusing scrapers + needles & syringe for total protein isolation -
(reply: 1)
478.
Trouble with equal loading -
(reply: 8)
479.
how many times striping can b done of the blot -
(reply: 9)
480.
SDS-PAGE of negatively charged protein -
(reply: 4)
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