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151. Left ECL kit at room temp. Is it ruined? - (reply: 2)
152. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
153. Native PAGE problem - not entering gel enough - (reply: 9)
154. film saturation in immunoblots - (reply: 1)
155. Problems with wells on SDS PAGE gels - (reply: 3)
156. Protein is present, but not detected by antibody - (reply: 4)
157. secondary antibody creation - (reply: 1)
158. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
159. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
160. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
161. Low signal, weird background stains on blot - (reply: 5)
162. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
163. Incomplete Blotting and staining of NC membrane - (reply: 7)
164. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
165. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
166. Help, protein ladder didn`t separate - (reply: 3)
167. Determining phosphorylation status of a protein by transfection - (reply: 5)
168. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
169. Problem with Denative lysis - (reply: 1)
170. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
171. Polyclonal antibody precipitating - (reply: 1)
172. manual 2D electrophoresis - (reply: 8)
173. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
174. sample sonication before loading - (reply: 6)
175. transfer of low molecular weight protein - (reply: 3)
176. How is the mass detected via SDS-PAGE? - (reply: 3)
177. Water in beads during boiling - (reply: 3)
178. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
179. Runing problems - (reply: 18)
180. Western Blotting of Amyloid Beta - (reply: 1)
152. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
153. Native PAGE problem - not entering gel enough - (reply: 9)
154. film saturation in immunoblots - (reply: 1)
155. Problems with wells on SDS PAGE gels - (reply: 3)
156. Protein is present, but not detected by antibody - (reply: 4)
157. secondary antibody creation - (reply: 1)
158. Are certain tissues prone to uneven loading? Issues with intestine. - (reply: 6)
159. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
160. high background with acetyl-lysine antibodies for IP immunoblot - (reply: 1)
161. Low signal, weird background stains on blot - (reply: 5)
162. Chemiluminescence vs. Fluorescence visualisation of western blot - (reply: 1)
163. Incomplete Blotting and staining of NC membrane - (reply: 7)
164. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
165. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
166. Help, protein ladder didn`t separate - (reply: 3)
167. Determining phosphorylation status of a protein by transfection - (reply: 5)
168. Will 10 min boiling disrupt BS3 cross linking? - (reply: 7)
169. Problem with Denative lysis - (reply: 1)
170. Effect of MeOH and acetic acid on primary-secondary complex - (reply: 3)
171. Polyclonal antibody precipitating - (reply: 1)
172. manual 2D electrophoresis - (reply: 8)
173. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
174. sample sonication before loading - (reply: 6)
175. transfer of low molecular weight protein - (reply: 3)
176. How is the mass detected via SDS-PAGE? - (reply: 3)
177. Water in beads during boiling - (reply: 3)
178. lysis buffer for generating lysate from ovarian and brain tumors? - (reply: 1)
179. Runing problems - (reply: 18)
180. Western Blotting of Amyloid Beta - (reply: 1)