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931. Native Page transfer/detection problems - (reply: 1)
932. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
933. nuclear loading control - (reply: 2)
934. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
935. Large protein transfer problem - (reply: 8)
936. overloading GAPDH but nice band of interest - (reply: 2)
937. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
938. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
939. Quantiying bands. - Determination of protein contens (reply: 2)
940. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
941. Strange lane pattern, yes/no pattern - (reply: 3)
942. Native PAGE w. Western protein marker - (reply: 4)
943. protein stuck - (reply: 3)
944. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
945. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
946. changing colour of front dye in sds-page - (reply: 10)
947. small protein blotting - (reply: 8)
948. how stable is alpha mouse HRP? - left on the bench (reply: 2)
949. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
950. Too many bands in my SDS-PAGE - (reply: 5)
951. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
952. Uneven bands in SDS-PAGE - (reply: 5)
953. Can primary antibody be reused ? - (reply: 6)
954. Preparation of tree tissue for western blot - (reply: 1)
955. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
956. direct lysis in Laemmli buffer - (reply: 4)
957. anti-FLAG (M2) cross reaction - (reply: 6)
958. NFkB western blotting - (reply: 3)
959. Mannitol in Lysis Buffer - (reply: 1)
960. how long will a 1M DTT solution will last in -20C - (reply: 5)
932. Why do I have to almost "burn" my transfer before I can detect?? - (reply: 26)
933. nuclear loading control - (reply: 2)
934. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
935. Large protein transfer problem - (reply: 8)
936. overloading GAPDH but nice band of interest - (reply: 2)
937. Lysis buffer for Immunoprecipitation - can I use detergents? (reply: 2)
938. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
939. Quantiying bands. - Determination of protein contens (reply: 2)
940. no front in sds-electrophoresis - no front is formed on my gel - why? (reply: 1)
941. Strange lane pattern, yes/no pattern - (reply: 3)
942. Native PAGE w. Western protein marker - (reply: 4)
943. protein stuck - (reply: 3)
944. What gel thickness and numebr of combs do you use? - and why?? (reply: 1)
945. SAMPLE MIGRATION PROBLEM IN sds PAGE - SAMPLE MIGRATION (reply: 9)
946. changing colour of front dye in sds-page - (reply: 10)
947. small protein blotting - (reply: 8)
948. how stable is alpha mouse HRP? - left on the bench (reply: 2)
949. Uneven leveling in resolving gel - SDS-PAGE resolving gel woes (reply: 6)
950. Too many bands in my SDS-PAGE - (reply: 5)
951. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
952. Uneven bands in SDS-PAGE - (reply: 5)
953. Can primary antibody be reused ? - (reply: 6)
954. Preparation of tree tissue for western blot - (reply: 1)
955. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
956. direct lysis in Laemmli buffer - (reply: 4)
957. anti-FLAG (M2) cross reaction - (reply: 6)
958. NFkB western blotting - (reply: 3)
959. Mannitol in Lysis Buffer - (reply: 1)
960. how long will a 1M DTT solution will last in -20C - (reply: 5)