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691. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
692. SDS PAGE- HELP - Bands not visible (reply: 4)
693. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
694. Strange SDS gel after Western Blotting - (reply: 1)
695. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
696. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
697. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
698. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
699. Western blot fail, time and time again - (reply: 21)
700. Western blot of urine samples - Problems verifying loading (reply: 1)
701. BSA test for native PAGE - (reply: 2)
702. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
703. transfer: amperes or volts? - (reply: 6)
704. dot blot works, western doesn't - HELP! - (reply: 5)
705. Liposome + IgG sample in SDS-PAGE - (reply: 1)
706. SDS-PAGE for proteins which have pI>7 - (reply: 1)
707. band diffusion in sds - (reply: 5)
708. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
709. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
710. Artifacts only in Reduced samples!!! - (reply: 18)
711. Over-current alarm - (reply: 1)
712. too much bands in western - (reply: 2)
713. PVDF membrane dried after transfer (Western) - (reply: 6)
714. Help Stained after blocking and won't unstain!!! - (reply: 1)
715. Bands are lower than actual protein - (reply: 1)
716. Protein lysate storage - (reply: 2)
717. Is there alpha-tubulin in the nucleus? - (reply: 4)
718. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
719. No band after WB revelation - (reply: 2)
720. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
692. SDS PAGE- HELP - Bands not visible (reply: 4)
693. Presence of 'blobs' at the dye front in SDS-PAGE - (reply: 2)
694. Strange SDS gel after Western Blotting - (reply: 1)
695. SDS-loading dye recipe - pH-sensitive loading dye recipe (reply: 4)
696. Irregularities in width of samples running on SDS-PAGE gel - (reply: 3)
697. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
698. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
699. Western blot fail, time and time again - (reply: 21)
700. Western blot of urine samples - Problems verifying loading (reply: 1)
701. BSA test for native PAGE - (reply: 2)
702. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
703. transfer: amperes or volts? - (reply: 6)
704. dot blot works, western doesn't - HELP! - (reply: 5)
705. Liposome + IgG sample in SDS-PAGE - (reply: 1)
706. SDS-PAGE for proteins which have pI>7 - (reply: 1)
707. band diffusion in sds - (reply: 5)
708. Is Histone H1 suitable to check the purity of cytoplasm extracts? - (reply: 1)
709. Determining whether the quality of an antibody and if it is species specific - (reply: 2)
710. Artifacts only in Reduced samples!!! - (reply: 18)
711. Over-current alarm - (reply: 1)
712. too much bands in western - (reply: 2)
713. PVDF membrane dried after transfer (Western) - (reply: 6)
714. Help Stained after blocking and won't unstain!!! - (reply: 1)
715. Bands are lower than actual protein - (reply: 1)
716. Protein lysate storage - (reply: 2)
717. Is there alpha-tubulin in the nucleus? - (reply: 4)
718. Cytoplasmic marker contamination for Nuclear extracts - Tubulin alfa or beta? (reply: 3)
719. No band after WB revelation - (reply: 2)
720. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)