Is immunoblotting possible after the gel has been silver stained - (Feb/03/2009 )
I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.
-Opal-
not likely to work. i would just run a duplicate for blotting.
-mdfenko-
Opal on Feb 3 2009, 03:27 PM said:
I have run some 2-D gels and have silver stained them. Now, I want to know which spot is my target protein; just wondering if I can western blot one of those 2-D gels. I am trying to stay away from running another 2-D.
Probably no as the protein was being fixed
-Nrelo-