Re-using membranes with ghost bands - (Oct/26/2011 )
Hi!
My question is if I can reblot a membrane with ghost bands again with the same antibody but less concentrate. Is this possible or I have to repeat the gel?
Thanks
Irene
-Irene G-
The Abs don't wash away. You would have to strip the membrane and then reprobe, but this is not very reproducible.
-BioMiha-
Thank you, I will make a new gel!
-Irene G-