Multiple bands on blot with one antibody - (Aug/06/2012 )
Ran a standard blot and saw 2 bands with a ~10kD difference (e.g. 125 and 130kD). Was wondering how do we know which band is the 'true' band? and is it common to see more than one band?
-science noob-
yes it's quite common to see non-specific bands. You just need to run controls to know which band is the true band.
-Curtis-