Acid urea gel electrophoresis (AUGE) - (Sep/04/2012 )

Dear friends,

I am doing AU and TAU gel electrophoresis for some basic proteins by Sandra Hake Method "Extraction, purification and analysis of histones" Nature Protocols (2007). 2(6): 1445-1457.
Since the basic proteins are positively charged so they move in the opposite direction as that of SDS.

What i dont understand is that the author is asking to switch the electrode leads in Acid urea gel electrophoresis so that the positively charged proteins run towards the cathode. How should i switch the electrode leads? either the orientattion of leads going in the power supply or the electrode leads going in the tank, or there is anything else. I am using BioRad Mini Protean III apparatus. please help me i am really confused.

Regards

Binsan

I used to run BAC gels which is a different type of acidic electophoresis, and all I did was I plugged the black lead into the red socket and the red one into the black socket and that worked just fine. I used a biorad apparatus too but I can't tell you what exact make they were, and they were big tanks for large gels.

I thought it was quite the fun to reverse the current.... Good luck!

oh thank you so much. you solved my problem

Regards
Binsan

to plug the black lead into the red socket and the red one into the black socket , BioRad Mini Protean III apparatus work well for that change?

yongzhi tian on Tue Jan 15 09:35:03 2013 said:

mdfenko on Tue Jan 15 19:15:11 2013 said:

is cytochrome c the only maker of AU gel? or anyone else?

are there any requirements for cytochrome c used by AU gel? could you recommend the very ones for AU gels? thanks!