How to load equal amount of protein without using spectramax - (Oct/18/2012 )

Hi,

I was going to do BCA protein quantification with spectra max, but that machine is not working.
And I am not sure what I can do without it.
Is there any way?

Thank you so much for your help.

You could still do the BCA protein quantification and run in parallel a known set of concentrations and then compare the color you get to your samples to get a "rough" estimation of protein concentration. Or you could run a SDS gel and load with known protein concentrations and compare your samples.

You can also load based on cell number if you are using cell culture lysates, I find it more reliable than BCA.

bob1 on Thu Oct 18 21:53:07 2012 said:

I am using the mouse liver. So after homogenize and extract protein, I was planning to do the BCA, but that spectra machine is not working.

If you have an ordinary spectrophotometer (even a nanodrop will work), you can do the assay using that, it is just a bit slower and you need to manually put the samples through rather than reading from a plate.