can RNA amplify in two-step qPCR? - (Mar/15/2011 )

Dear all,

I wonder if RNA (not cDNA) be amplified in 2-step qPCR? Or there is jus contamination! I am using KAPA™ SYBR® FAST qPCR Kit.

tkmz on Tue Mar 15 13:38:46 2011 said:

So if I suspect there is a contamination in my cDNA after running NTC in the qPCR, I can rule out RNA contamination. Thanks!