RNA agarose gel blurry fuzzy. RNase contaminaton? - (Nov/17/2015 )
I extracted total RNA from a PNT2 cell line and ran on an agarose gel. The two major rRNA bands appear in the 1-2kb regions but look blurry and fuzzy. Is this a sign of RNase-contamination, simply overloaded, or is it ready for downstream experiments? Shown on the left side is a 2-log ladder standard.
-qpwoei4756-
I don't think that is RNase contamination
-Curtis-
It looks that you have RNA degradation and depending of the size of the molecular ladder the top band could be DNA contamination.
-merlav-