Too many bands in my SDS-PAGE - (Sep/17/2009 )

Hello,
can someone please help out, I suddenly have started getting many bands in my SDS-PAGE after staining and destaining. I made sure that my comb was clean and my gel plates were properly washed and cleaned but I still got these bands after running SDS-PAGE analysis. Previously I got only bands for my target protein but now I have these unwanted bands.
Please any suggestions about how to get rid of these unwanted bands?

Thank in advance

-ojbillions-

Gels run using whole cell lysate? purified protein?

-bob1-

one of your buffers is probably contaminated.

-mdfenko-

Or Ab going old!

-madrius1-

One possible reason is sample degradation.

Or, did you change the voltage from the power supply? If you use a lower voltage, it is possbile that the previously unresolved bands are separated (low V, high resolution).

-kristian-

Hi ojbillions, welcome to the BioForums!

To answer your question, we need more information about your procedure. When you say "bands", do you mean bands on a stained PAGE gel or bands on a western blot of a PAGE gel? What is the nature of the sample you're loading -- is it a whole cell lysate or a purified protein sample?

-HomeBrew-