overloading GAPDH but nice band of interest - (Oct/13/2009 )

I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?

-sllwestern-

sllwestern on Oct 13 2009, 11:38 AM said:

I am trying to probe for endogenous myc which is at very low concentrations in the nucleus. I now have a band for myc, but my GAPDH is smeared on the membrane. Any suggestions for non-smeared GAPDH?

What about using much less primary? or load two samples at 1x and 10x and stain for myc and GAPDH respectively.

-Dr Teeth-

Or perhaps use a shorter exposure if developing film/digital images

-bob1-