stability of the stripping buffer - (May/14/2010 )
Hi!
I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.
Thanks
-AnaG-
AnaG on May 15 2010, 01:56 AM said:
Hi!
I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.
Thanks
I would like to know for how long the stripping buffer (with beta-mercaptoethanol) is stable. I prepared it about two weeks ago and kept it (at room temperature) but now I don't know if I can use it or if I should prepare a new solution.
Thanks
well we always use a fresh one wen needed so not sure about the stability but i wud suggest if u not running short of the components prepare fresh.. alternatively try to smell if the mercapto is still tere or gone!!!
-Prep!-
two weeks at room temperature is too long for mercaptoethanol.
make fresh.
-mdfenko-