downshifted band - (Apr/25/2010 )

When I load a cell lysate on a gel and do western blot against my protein of interest, I see a band at the right size(56kDa) and another that's about 10kDa lower.

When I perform an siRNA knockdown the lower band also disappears.

I know many modifications causing an upshift, but what can be causing any downshifts?

Thanks for the suggestions in advance

-cellgene-

Potentially cleavage of a fragment.

-bob1-

cellgene on Apr 25 2010, 08:20 PM said:

proteolysis or splice variation or loss of posttranslational modifications

-Inmost sun-

thank you
i couldn't find any cleavage sites bioinformatically- but of course it still can be

So no modification causes the protein to run lower?

-cellgene-

cellgene on May 5 2010, 08:37 AM said:

thank you
i couldn't find any cleavage sites bioinformatically- but of course it still can be

So no modification causes the protein to run lower?

an example, carboxypeptidases will remove one amino acid at a time, no specific cleavage site other than it be the carboxy terminus.

cleavage can be a ptm.

-mdfenko-