SDS page problems - (Jun/27/2009 )

the dying protein appear a distrubution cuve when I run the gel ..

I do see a buble in the middle of my gel, but I wonder why it happen..

because bubbles in the champer or there is gaps between the acrylic pressure plate and the longer glass plate?

thanks for help..

I am not sure if I understand yr question. Can you give more details?