western without tween - (Feb/21/2011 )

hi,
I forgut to put tween while doing the western blot. will it change the results drastically?

Not really. You could observe slightly more non-specific binding but I wouldn't worry about it too much.

nataly on Mon Feb 21 08:15:28 2011 said:

you might have more background and/or non specific bands, or not, depending on your antibody.
good luck

thanks