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Methylation Related Discussions
  1. methylation specific PCR - new to epigenetics-where to start (reply: 1)
  2. About DNMT1 antibody - (reply: 1)
  3. MSP primer does not work - (reply: 5)
  4. strang phenonmenon in MSP experiment - (reply: 2)
  5. How to increase the product amount in BSP? - weak amplification in BSP (reply: 3)
  6. restriction enzymes that cut at methylated cytosines - (reply: 2)
  7. where to buy universally methylated and unmethylated genomic DNA? - (reply: 7)
  8. methylnick.please help me with MSP primer designing! - (reply: 3)
  9. dam methylation? - digestion problem (reply: 3)
  10. No Product from MethylEasy Kit Bisulfite Treatment! - (reply: 3)
  11. Advantages of ChIP vs. sodium bisulfite sequencing - (reply: 2)
  12. About DAPK1 MSP primer design. - About MSP primer design. (reply: 4)
  13. protein/DNA methylation interaction - (reply: 5)
  14. Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
  15. Visualizing CpG island in IF - Are there antibodies available? (reply: 1)
  16. methylation specific PCR - (reply: 5)
  17. Bisulfite sequencing reproducibility - overlapping PCR amp primers give varying results (reply: 2)
  18. Please help me design MSP primer! - (reply: 4)
  19. Low MSP sensitivity - (reply: 1)
  20. MSP gives smear only - (reply: 4)
  21. Quantifying Bisulfite treated DNA - (reply: 7)
  22. Infinium MEthylation Array Validation - (reply: 1)
  23. panomics promoter methylation PCR kit - results not what i expected (reply: 1)
  24. A question about MSP - (reply: 4)
  25. Methylation troubleshooting - (reply: 7)
  26. Amplicon size for BSP - (reply: 3)
  27. Primer dimer problem in MSP - (reply: 2)
  28. DNA methylation analysis - doubts - (reply: 5)
  29. methylation of plasmids - (reply: 1)
  30. Panomics Promoter Methylation PCR Kit and Methylation specific Digests - (reply: 2)
  31. question about MSP bands - (reply: 7)
  32. methylated plasmids in culture - (reply: 1)
  33. newbie question on Primer design for MSP - (reply: 2)
  34. Confirming published methylation primers - (reply: 1)
  35. Problems with Bisulfite PCR - (reply: 13)
  36. Cloning with methylated vector - (reply: 2)
  37. methylation affects ligation? - (reply: 2)
  38. Bisulfite PCR Question - (reply: 2)
  39. PCR bias with BSP primers - (reply: 1)
  40. CpG island prediction - (reply: 3)
  41. Sequencing of MSP products? - (reply: 5)
  42. complete bisulfite treatment - How do you know if your DNA is completely modified by bisulfite treatm (reply: 2)
  43. MSP - (reply: 3)
  44. MSP is not reliable! - methodology discuss. (reply: 4)
  45. DNA methylation - (reply: 5)
  46. MethyISEQr Bisulfite Conversion kit from AB, is it good? - (reply: 4)
  47. Site specific Bisulfite analysis Problem - (reply: 3)
  48. How long does the methylation status of a gene last after AZA treatment? - (reply: 2)
  49. methylated restriction sites - (reply: 1)
  50. please help me check BSP primers. - (reply: 6)
  51. a problem about the Tm of the BSP primer - (reply: 2)
  52. How to study promoter. - A presentation in related to DNA methylation (reply: 1)
  53. who can give me a hand to check the BSP primer? - (reply: 5)
  54. Please help me design BSP primer. - (reply: 14)
  55. bisulfite PCR: is it my product? - (reply: 5)
  56. Please help me analyze BSP cloned sequencing results. - (reply: 8)
  57. normalization in MSP with heterogenous sample - (reply: 1)
  58. DNA Methylation Analysis - MeDIP (reply: 5)
  59. what is nethylation? - not methylation but nethylation (reply: 5)
  60. DNA quality for MSP? - (reply: 2)
  61. RT PCR for methylation analysis - (reply: 3)
  62. Bisulfite Sequencing: establishment - (reply: 1)
  63. kit for bisulfite treatment - (reply: 3)
  64. DH5 alpha methylation profile - (reply: 8)
  65. quantitative methylation analysis? - (reply: 7)
  66. WGA problems CpG island amplification - (reply: 6)
  67. methylation analysis - methylation analysis using epitect bisulfite kit from qiagen (reply: 11)
  68. need a whole genome methylation protocol - (reply: 2)
  69. ***MSP Primer Troubleshooting Specificity*** - (reply: 6)
  70. Methylation studies - New to this field... help me (reply: 1)
  71. Bisulfite direct sequencing + cloning - (reply: 2)
  72. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
  73. DNA methylation with dimethyl sulphate - (reply: 1)
  74. Inconsistent bisulfite PCR - (reply: 8)
  75. Serious problem with bisulfite modification - (reply: 3)
  76. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
  77. Bisulfite sequencing condition - (reply: 3)
  78. Bisulfite tx'd DNA sequence analysis - what to do with CG to TA conversion - bisulfite sequencing analysis (reply: 7)
  79. software for CpG island prediction for a list of genes - (reply: 3)
  80. Can bisulphite treatment be used for detection of DNA methylation within bacteri - (reply: 2)
  81. Does a gene have an associated CpG island? - (reply: 9)
  82. DNA methylation in leucocyte - (reply: 2)
  83. Problems with TA cloning of bisulfite converted PCR product - (reply: 2)
  84. Duplex MSP? - (reply: 2)
  85. When Methylation Results are not Reproducible - (reply: 1)
  86. Wrong PCR product after Bisulfite Treatment-reason? - (reply: 1)
  87. A question about sample preservation for BSP - (reply: 2)
  88. Bisulfite Modification Efficiency - (reply: 5)
  89. MSP: M and U primers products - (reply: 1)
  90. Advice on analysing Differentially Methylated Regions of a gene. - (reply: 5)
  91. CpG island and promoter - (reply: 3)
  92. ready to use primers for BSP - (reply: 4)
  93. How to association the methylation profiling with the expression? - statistic questions about the bisulfite sequencing (reply: 1)
  94. DNA methylation direct kit - (reply: 2)
  95. direct bisulfite sequencing - (reply: 6)
  96. Negative control shows methylation? - (reply: 1)
  97. Free Minipreps and 50% off + Free Sequencing Clean-Up + Methylation Beta Kits - Free kit offers (reply: 3)
  98. MSP and BSP confusion - a bit confusion about MSp (reply: 4)
  99. Real-Time MSP - (reply: 1)
  100. primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
  101. Methylated cytosine nucleotide - (reply: 1)
  102. Bisulfite sequencing - why cloning? - (reply: 3)
  103. what is the best dna methylation/modification kits? - newbie kit comparison (reply: 18)
  104. Illumina Golden gate methylation? - (reply: 4)
  105. help pls: general qs about methylation - (reply: 7)
  106. Nested PCR for BSP question - (reply: 4)
  107. Overcoming PCR bias before bisulfite sequencing?! - (reply: 2)
  108. MSP Primer design -rules to choose primers and reasons - (reply: 2)
  109. Why are CpG sites more prone to mutation? - (reply: 4)
  110. pGEM methylation status - (reply: 6)
  111. Bisulfite modification of DNA from paraffin embedded tissue - (reply: 4)
  112. QPCR with MSP - (reply: 3)
  113. MSP primer design - understanding the basics - (reply: 2)
  114. Bisulfite: PCR condition or primer problem? - (reply: 13)
  115. Real-time PCR to analyze DNA methylation - (reply: 3)
  116. Nanog Methylation and sequencing - (reply: 3)
  117. Preferential methylation of specific CpG sites? - (reply: 9)
  118. Bisulfite sequencing of PCR products after AIMS technique - (reply: 3)
  119. Global DNA methylation - [3H]dCTP method - (reply: 2)
  120. tool for methylation - (reply: 1)
  121. Methods Comparison for Methylation Analysis - Which method is the best? (reply: 5)
  122. Cloning for Bisulfite sequencing - TOPO TA no results...... pGEM???? (reply: 2)
  123. PCR of methylated DNA? - (reply: 1)
  124. ELISA/Dot blot for detection of Methylated DNA - (reply: 3)
  125. systemic way to reduce BSP PCR bias - (reply: 1)
  126. TATA BOX AND CPG ISLANDS - (reply: 1)
  127. What kit to use for bisulfite sequencing? - (reply: 1)
  128. methylation, irreversible? - (reply: 2)
  129. Troubleshooting with Bisulfite - (reply: 4)
  130. Gene body methylation and active gene expression - (reply: 6)
  131. Trouble repeating MSP protocol - (reply: 1)
  132. DNA Methylation - Tissue specific - (reply: 4)
  133. BSP - which polymer do you use? - Which sequencing polymer gives the best results? (reply: 1)
  134. De novo plasmid methylation in eukaryotic cells - (reply: 3)
  135. Melting Curve Analysis for semiquantitative measurement of promoter methylation - (reply: 5)
  136. type of detection, type of bisulfite conversion, =>different results? - variability between preps and such (reply: 9)
  137. Question about BSP primer design? - What is the complementary strand for forward primer? (reply: 3)
  138. Good or not?----MethylDetector™ Bisulfite Modification Kit - Could anyone help me evaluate this protocal for bisulfate treatment (reply: 2)
  139. Quantitative Assay for DNMT1 activity... - need help please (reply: 11)
  140. Controls for Bisulfite Sequencing - (reply: 13)
  141. False priming in MSP - (reply: 4)
  142. False positives? MSP and BGS - (reply: 9)
  143. Panomics Methylation Promoter PCR Kit - (reply: 1)
  144. bisulfite treatment - in my hands bisulfite kit don't work well (reply: 1)
  145. Nested MSP? - (reply: 3)
  146. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  147. Methylation studies using arrays - (reply: 7)
  148. Methylation of promoter-luciferase construct - investigate methylation on promoter activity (reply: 7)
  149. Qiagen EpiTect - Kit - No PCR product after BSP, only smear! (reply: 11)
  150. Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
  151. DNA from somatic tissues for bisulfite treatment - (reply: 3)
  152. Does anyone know any kind of array or chip for methylation scan? - (reply: 3)
  153. Scoring CpG methylation by peak height - (reply: 4)
  154. Nanodrop question - Spectrophotometrical measurment of bisulfite treated DNA (reply: 15)
  155. Plasmid CpG methylation after transfection - (reply: 2)
  156. Direct sequencing on BSP PCR products: problems with A and T stretches - (reply: 3)
  157. Protocol for DNA Methylation analysis using restriction enzyme digest - (reply: 10)
  158. Methyl Primer Express from ABI - (reply: 2)
  159. Is it absolutely necessary to do BGS before doing MSP for new genes? - (reply: 4)
  160. ChIP and CpG island microarrays - (reply: 1)
  161. Methylated DNA Interfering with Restriction Digestion - Restriction digestion problems (reply: 3)
  162. MSP/USP in human tissue - USP not working! (reply: 3)
  163. A new protocol:High-speed conversion of cytosine to uracil in bisulfite genomic - (reply: 6)
  164. General methylation question - (reply: 1)
  165. MSP for help - (reply: 10)
  166. Primer design for BSP - (reply: 12)
  167. Site Directed Mutagenesis within CpG rich promoter - (reply: 4)
  168. HMW band in well after bisulfite PCR - (reply: 1)
  169. Methylation specific primer designing - (reply: 1)
  170. 5ug DNA can be converted in one bisulfite treatment - (reply: 8)
  171. MSP BUFFER BY Herman et al., - (reply: 2)
  172. Problems with PCR following bisulfite treatment - (reply: 11)
  173. promoter methylation PCR kit from Panomics - (reply: 2)
  174. Heavy BSP bias towards methylated DNA - Why??? (reply: 5)
  175. MSP question - (reply: 1)
  176. Which CpG island to pick? - (reply: 1)
  177. Bis. PCR not working. MSP is fine - (reply: 1)
  178. where to find the list of genes known to be methylated - (reply: 2)
  179. A problem with bisulfite sequencing - (reply: 7)
  180. No PCR Product after Bisulfite Modification - (reply: 5)
  181. about the primers in MSP - (reply: 5)
  182. How to transfer the BSP sequencing results into the standard legend? - (reply: 8)
  183. Must I do MSP focused on survivin gene's exon 1 . - (reply: 2)
  184. Bisulfite Sequencing Technique - (reply: 8)
  185. Sequencing of bisulfite treated DNA - (reply: 7)
  186. BSP question - (reply: 2)
  187. HOW to explain my MSP result of negative control? - (reply: 9)
  188. Genome-wide methylation analysis - (reply: 4)
  189. Direct BSP using tag-modified primers - (reply: 2)
  190. start a methylation study - (reply: 10)
  191. help, methylated CMV promoter inactivated - (reply: 3)
  192. need help with methylation pattern - (reply: 5)
  193. Question about bisulfite PCR - (reply: 2)
  194. MSP and Bisulfite genomic sequencing results don't match! - (reply: 5)
  195. Calculating Tm for bisulfite primers? - (reply: 3)
  196. DNA methylation and gene silencing - (reply: 5)
  197. real-time PCR for quantitation of MSP - (reply: 3)
  198. methylated plasmid - (reply: 3)
  199. Methylation of DNA, isolated from whole blood. - (reply: 1)
  200. ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
  201. MSP primer design help - (reply: 5)
  202. Unique unspecific product using MSP - (reply: 3)
  203. BSP and MSP Primers - (reply: 2)
  204. Are transiently transfected DNA methylated in mammalian cells? - (reply: 3)
  205. How to isolate DNA from 1-5 blastocyst or oocytes for methylation studies - (reply: 2)
  206. Bisulfite sequencing primer - (reply: 4)
  207. Does MSP need sequence the product ? - (reply: 2)
  208. MSP Primer Sets (Herman Papers) - (reply: 1)
  209. methylation in patient samples - (reply: 1)
  210. BSP, MSP - many different ways - (reply: 4)
  211. Must all BSP be nested PCR? - (reply: 4)
  212. Methylated + bisulfite-treated DNA works better than w/o methylation - Desulfonation responsible? (reply: 8)
  213. some questions in DNA methylation - (reply: 2)
  214. DNA methylation vs gene expression - (reply: 3)
  215. XbaI/BamHI double digest- dam methylation detection problem - (reply: 3)
  216. BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
  217. MSP question - (reply: 1)
  218. Bisulfite sequencing results - how to analyze (reply: 1)
  219. transposon methylation and gene silencing - (reply: 9)
  220. Bisulfite treatment? - (reply: 1)
  221. MSP for formalin-fixed and paraffin-embedded tissues - (reply: 2)
  222. Positive Control for Methylation Studies in BSP sequencing - A "Universally" methylated Gene ?? (reply: 7)
  223. designing primer for methylation - (reply: 21)
  224. MSP - (reply: 1)
  225. about the methylation content (MSP) - (reply: 11)
  226. MSP PCR - (reply: 2)
  227. unexpected bands on MSP - (reply: 2)
  228. Puzzle about methylation on plant microRNA - (reply: 1)
  229. from which strand to derive BSP primers? - (reply: 5)
  230. Success with Direct Sequening of Bisulfite Pcr Products - (reply: 47)
  231. Is it possible to use ChIP-chip for identifying CpG island methylation? - (reply: 10)
  232. How long can Bisulfite treated DNA be stored? - (reply: 6)
  233. Sodium bisulfite composition - (reply: 3)
  234. MSP buffer composition - (reply: 1)
  235. Direct bisulfite sequencing of PCR-products: degree of CpG methylation - (reply: 4)
  236. CpG calculation? - (reply: 3)
  237. Find antidoy for EZH2 EED SUZ12 for doing CHIP - histone methylation (reply: 2)
  238. MSP condition - (reply: 4)
  239. DNA methylation analysis of the target gene - (reply: 2)
  240. MSP trouble - (reply: 4)
  241. Can't get MSP PCR products at all after treatment! - Help with MSP using Chemicon Fast DNA Mod Kit (reply: 1)
  242. Trouble with primers of BSP - (reply: 3)
  243. Touchdown PCR for MSP? - (reply: 4)
  244. DNA methylation content (degree) - (reply: 2)
  245. What is the advantage of nest PCR in methylation analysis - (reply: 1)
  246. Two bands with MSP - (reply: 6)
  247. Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
  248. How to determine the DNA methylation of the whole gene - Primer design for BSP (reply: 18)
  249. Problems with bisulfite sequencing - (reply: 11)
  250. Methylation Analysis-New methods? - (reply: 7)
  251. Control DNA in bisulfite methylation analysis - (reply: 5)
  252. shorter PCR products after bisulfite treatment and cloning - (reply: 5)
  253. Do I need internal controls in MS-PCR? - Methylation specific PCR Internal controls (reply: 3)
  254. whole blood for dna methylation? - (reply: 2)
  255. How to extract DNA from fixed pathologic specimens for MSP - (reply: 5)
  256. How to eliminate primer dimer in MSP - (reply: 20)
  257. Prokaryotic/eukaryotic DNA methylation review article - (reply: 14)
  258. The sensitivity of MSP - (reply: 1)
  259. cpg as an adjuvant - cpg procurment (reply: 3)
  260. CpG island definition - (reply: 1)
  261. CpG islands and start sites - (reply: 1)
  262. Nested MSP - (reply: 3)
  263. methylation Primers - Methyalted PCR (reply: 1)
  264. BSP - favors amplification of unmethylated template? - (reply: 2)
  265. Methylation Confusion and Headaches - (reply: 1)
  266. Which polymerase for bisulfite sequencing PCR - (reply: 3)
  267. non-CpG methylation - (reply: 3)
  268. Starting to work with methylation - (reply: 1)
  269. inner primer for BSP PCR? - (reply: 6)
  270. Methylation and expression of imprinted genes - (reply: 3)
  271. Bisulfite conversion - necessary to digest DNA? - (reply: 1)
  272. global methylation level - (reply: 2)
  273. EZ DNA methylation kit is not working anymore - (reply: 4)
  274. Mouse MSP primers - (reply: 11)
  275. DNA methylation in Raji cell line - (reply: 1)
  276. question after getting BSP result - (reply: 2)
  277. Two round MSP with the same primer - (reply: 1)
  278. HPLC protocol for methylation quantification - (reply: 21)
  279. BSP in repetitive region - (reply: 3)
  280. Help!Checking the nested Primer - P15 gene nested MSP (reply: 2)
  281. direct sequencing of bisulfite modified DNA - (reply: 5)
  282. How to determine the result of my msp? - methylated or unmethylated? (reply: 5)
  283. bisulfite sequencing: cell lines vs tumors - (reply: 3)
  284. p16 methylation in pituitary adenomas - p16 in pituitary adenomas (reply: 2)
  285. Methylation Workshops? - (reply: 1)
  286. What the hell is CpG island methylator phenotype (CIMP)? - (reply: 6)
  287. No CpG island=methylation is not important for regulation? - (reply: 7)
  288. MSP result - (reply: 3)
  289. method of using the CpG methylase enzyme - (reply: 7)
  290. Which MSP & BGS primer design tool do you "swear" by? - (reply: 9)
  291. Partial methylation/incomplete modification – BSP - (reply: 3)
  292. Which DNA fragment as control for complete bisulfite conversion - (reply: 6)
  293. Methylation & PCR - (reply: 1)
  294. MSP product size - (reply: 1)
  295. Dnmt1 antibody - (reply: 1)
  296. How to tell incomplete bisulfite treament from real methylation - (reply: 1)
  297. primer design for MSP - How many CpG in the primer? (reply: 2)
  298. Does methylation matter? - (reply: 1)
  299. Steps for the BSP - (reply: 1)
  300. MSP question - (reply: 1)
  301. No PCR products in MSP with MethPrimer designed primers - (reply: 2)
  302. Allele specific cloning efficiency after bisulfite treatment - (reply: 4)
  303. Questions about MSP - (reply: 1)
  304. Do you believe CpG methylation pattern? - (reply: 7)
  305. bisufite converted DNA sequence - (reply: 5)
  306. multiple products after MSP - (reply: 6)
  307. problems with mix of MSP's primers - (reply: 2)
  308. CpG methylation - (reply: 7)
  309. Positive control for bisulfite converted genomic DNA? - (reply: 1)
  310. Southern blot v. bisulfite sequencing - which is better to detect methylation? (reply: 1)
  311. Bisulfite DNA modification protocol - (reply: 6)
  312. CpG methylation - Methylation on GpC also possible? (reply: 2)
  313. From MSP primer to gene - how to recalculate original DNA sequence? (reply: 4)
  314. MSP primers not working - (reply: 1)
  315. No PCR product after BSP, but dimers - (reply: 5)
  316. problems with bisulfite PCR - (reply: 8)
  317. How To Make 2M Bisulfite Solution? - Bisulfite Sequencing (reply: 6)
  318. methylation sensitive restriction site - (reply: 2)
  319. How to control MSP contamination - (reply: 3)
  320. About MSP result ! - why always have two bands (reply: 3)
  321. How long did you treat DNA with bisulfite? - (reply: 1)
  322. Non CpG cytosine methylation - Global methylation analysis of somatic tissue (reply: 1)
  323. how to reduce the dimer in MSP - (reply: 7)
  324. Does it help to add antisense primer first for bisulfite PCR? - (reply: 2)
  325. CpG island - Definition (reply: 6)
  326. Bisulfite treated DNA specific primer set critique? - (reply: 18)
  327. true methylation target - downstream of a true methylated gene (reply: 2)
  328. No product/primer dimers after BSP - (reply: 1)
  329. bisulfite modification after pcr - (reply: 2)
  330. interesting flower - plant methylation (reply: 4)
  331. Problems with methylation analysis!!! - Primers crossreaction etc. (reply: 3)
  332. Bisulfite genomic sequencing - Bisulfite genomic sequencing (reply: 1)
  333. Length of the BSP product - (reply: 1)
  334. Unpurifed DNA for methylation - (reply: 1)
  335. how to calculate the percent methylated reference (PMF).? - (reply: 4)
  336. One 'T' missing on one allele after bisulfite treatment - (reply: 8)
  337. Bsp: condition optimizing - (reply: 3)
  338. bisufite treated products - (reply: 5)
  339. designing flanking primers around msp primers - (reply: 1)
  340. Primer for BSP - (reply: 1)
  341. methylation vary with passage number? - (reply: 4)
  342. BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
  343. No band on BSP - (reply: 17)
  344. Troubleshooting problem in PCR for bisulfite Treated DNA - (reply: 1)
  345. No PCR product after bisulfite treatment :( - (reply: 3)
  346. Does Temperature Alter DNA Methylation Status - Temperature and Methylation (reply: 1)
  347. how can I represent CpG site as bar? - (reply: 2)
  348. Genes with no CpG islands... - (reply: 6)
  349. can we quantify bisufite treated DNA - (reply: 3)
  350. what is genomic DNA size after bisulfite treatment? - (reply: 3)
  351. How to do --- MSP or Clone-sequencing? - (reply: 1)
  352. Can these CpG islands be representative? - (reply: 1)
  353. How to explain my MSP sequencing result? - (reply: 6)
  354. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  355. Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
  356. MSP bands - both in methylated and unmethylated DNA (reply: 3)
  357. wrong sized bands/smear in both nested MSP PCRs - (reply: 5)
  358. General CpG island questions - (reply: 15)
  359. How can I know complete bisulfite conversion - (reply: 2)
  360. Help: about the MSP result ! - how to explain the My MSP result? (reply: 1)
  361. How to setup control for bisulfite sequencing PCR - (reply: 2)
  362. About MSP positive control - Choose what to methylate as positive control (reply: 5)
  363. How to find promoter regulation region CpG islands - (reply: 2)
  364. Storage Of Bisulfite treated DNA - Storage conditions (reply: 2)
  365. Bisulfite Sequencing Primer Design - (reply: 3)
  366. Starting with DNA methylation analysis - (reply: 11)
  367. Methylation of housekeeping genes? - (reply: 3)
  368. methylation in CpG island - (reply: 5)
  369. Methylation found in normal but not in the paired tumor..WHY - (reply: 16)
  370. Why proofreading Taq can't be used in MSP - (reply: 6)
  371. CpG site or CpG island, which is important - (reply: 23)
  372. Problems with MSP - Unable to pick up bands for methylation (reply: 1)
  373. Statistical analysis of methylation - (reply: 2)
  374. Plasmid methylation problem - Plasmid methylation (reply: 3)
  375. Storage of bisulfite modified DNA - (reply: 4)
  376. Reading chromatograms after bisulfite sequencing - Bisulfite sequencing (reply: 7)
  377. DNA Methylation in vitro - How to carry out DNA methylation on a given oligo (reply: 3)
  378. Analysis of CpG islands and promoters for methylation study - (reply: 10)
  379. The perfect number of CpGs in MSP primer - (reply: 8)
  380. Different methylation with normal Taq and High fidelityTaq - Should I use non-fidelTaq instead of High fidelity (reply: 4)
  381. taqman probe design for MSP - (reply: 3)
  382. Bisulfite sequencing -- Positive control? - bifsulfite sequencing (reply: 3)
  383. Cleaning up genomic DNA for bisulfite modification - (reply: 14)
  384. DNA extraction for methylation - DNA from cell-free serum for methylation treatment (reply: 4)
  385. MSP data presentation - What would be the best way to show MSP data (reply: 5)
  386. CpG islands in complement - (reply: 1)
  387. protein arginine methylation in E. coli? - (reply: 2)
  388. CpNpG - methylation or incomplete conversion? - (reply: 2)
  389. Bisulfite sequencing - (reply: 13)
  390. Why can the MSP represent the methylation of CpG island? - (reply: 3)
  391. How amplify long fragment from bisulfite treated DNA for MSP - (reply: 2)
  392. Multiplex MSP? - (reply: 4)
  393. distinguish between T and C after bisulfite sequencing - (reply: 3)
  394. Folate And Methylation - (reply: 56)
  395. Predict CpG island - (reply: 3)
  396. Designing methylation primers on long CpG island - (reply: 14)
  397. Suppression Subtractive Hybridization for methylation study - (reply: 1)
  398. Bisulfite sequencing problems - T>C conversion (reply: 13)
  399. real time PCR primer for methylation - (reply: 2)
  400. Define a CpG island - (reply: 2)
  401. How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
  402. bisulphite treatment for MSP - bisulphite treatment for MSP (reply: 7)
  403. Differences between MSP and BSP? - (reply: 37)
  404. Bisulfite sequencing problems - when CpG sites are too much... (reply: 4)
  405. How to search and retrieve promoter sequence for methylation or ChIP study - (reply: 17)
  406. Amplify long fragment from bisulfite treated template - for bisulfite sequencing (reply: 19)
  407. Creating CpG dinucleotide map across a promoter - DNA methylation and Bioinformatic anaylsis (reply: 8)
  408. mC not in CpG? Primer selection - (reply: 1)
  409. CpG island identification - (reply: 1)
  410. Enzyme digestion before bisulfite treatment - (reply: 1)
  411. methylation enzymes - (reply: 1)
  412. I want to learn "MSP" - (reply: 2)
  413. Online tools for methylation study - (reply: 11)
  414. ranting about methylation analysis - (reply: 2)
  415. Problems with BSP using Methprimer - (reply: 1)
  416. Starting methylation analysis, how! - (reply: 1)
  417. Sequence input for bisulfite PCR primer design - (reply: 1)
  418. Primer design for MSP queries - (reply: 3)
  419. DNA denaturing problem for bisulfite sequencing - (reply: 1)
  420. Is there a PCR length limitation after Bisulfite treatment? - (reply: 3)
  421. Bisulfite sequencing PCR problem - no products (reply: 3)
  422. The actual bisulfite treatment - protocol and troubleshooting (reply: 58)
  423. methylated genomic DNA PCR - (reply: 1)
  424. PCR more difficult in methylated CpG region? - (reply: 7)
  425. DNA methylation kit - (reply: 1)
  426. Assessing methylation levels - not CpG specific but across CpG island (reply: 3)
  427. CpG island sequence examples - database (reply: 1)
  428. Negative control for MSP - (reply: 4)
  429. partially methylated? - (reply: 1)
  430. why don't do MSP product sequencing - (reply: 2)
  431. BSP and MSP primer check - (reply: 2)
  432. Problems with bisulfite modification - Problems with bisulfite modification (reply: 3)
  433. DNA methylation - (reply: 3)
  434. Methylation difficulties - (reply: 6)
  435. Overall methylation - (reply: 5)
  436. MSP - specificity - (reply: 2)
  437. availabre primers for MSP - (reply: 1)
  438. Bisulfite sequencing - Does strand matter?? (reply: 3)
  439. Bisulfite sequencing and PCR issues - (reply: 1)
  440. positive control for MSP - (reply: 3)
  441. how to measure global methylation changes - (reply: 1)
  442. DNA hypo-methylation analysis - Check if a CpG island is hypomethylated (reply: 1)
  443. bisulfite kit - which one? - quality, price, quantity (reply: 4)
  444. methylation primers - problems with primers (reply: 1)
  445. Candidate gene methylation analysis - Some advice needed! (reply: 2)
  446. Methylation and restriction digestion problem - (reply: 8)
  447. Method to look for novel methylated gene - (reply: 3)
  448. Bisulfite modification kits and PCR - (reply: 5)
  449. Problems in bisulfite sequencing reproducibility - (reply: 7)
  450. PCR Cloning - primer design and CpG methylation (reply: 4)
  451. How short can be the CpG island? - methylation (reply: 3)
  452. CpG Island length variation - How variable? (reply: 3)
  453. Bisulfite PCR: Smear - Can't amplify a certain sequence (reply: 4)
  454. bisulfite pcr primers - (reply: 1)
  455. bisulfite modification of dna for pcr - (reply: 1)
  456. methylation specific PCR (MSP) - reproducibilty? (reply: 1)
  457. Perl program for CpG island prediction - where can I find source code (reply: 2)
  458. Software for MSP (methylation-specific PCR) primer design - (reply: 3)