ChIP and CpG island microarrays - (Oct/20/2006 )
Hello
Can anyone clarify for me if ChIP must be performed before CpG island microarrays are conducted on genomic DNA?
Thanks
-MDR-
Either Chip or MeDIP. Chip is chromatin immunoprecipitation, you can however perform methylated DNA IP (MeDIP) which uses an antibody against 5mC. Alturnatively it is possible to perform a methylsensitive restriction digest along with the methyl insensitive isoschizimer, isolate appropriate size range from both and the co hybridize.
N
-methylnick-