true methylation target - downstream of a true methylated gene (Aug/17/2005 )

Hi all,

I have successfully restored a gene following treatment of a methylation inhibitor, however, MSP showed it is unmethylated. This indicated that it could be downstream of a true methylated gene. I wonder if anyone has any suggestions on how to find the upstream methylated gene? Thanks!

unsure if you mean upstream of a biochemical pathway or physically 5' upstream of the gene??

Hi Nick

I meant upstream of a biochemical pathway. Thanks.