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Enzyme Related Discussions
  1. Enzyme inhibition - (reply: 1)
  2. restriction enzyme digestion - (reply: 5)
  3. restriction enzymes that cut at methylated cytosines - (reply: 2)
  4. DNA enzyme digestion problems - Smeared and stuck in wells, no or faint bands (reply: 1)
  5. SapI enzyme - (reply: 3)
  6. screening for enzyme activity - (reply: 3)
  7. Enzyme assay - (reply: 1)
  8. Pulse Field Gel Electrophoresis (PFGE) - Secondary Restriction Enzyme Problem (reply: 2)
  9. Enzyme extraction/isolation - (reply: 2)
  10. genomic library - use of restriction enzymes (reply: 10)
  11. Restriction enzymes not cutting - (reply: 4)
  12. Restriction Enzyme digestion - (reply: 4)
  13. Radioenzymatc Assay - How to convert cpm values into enzyme specific activity (reply: 1)
  14. ENZYME ASSAY WITH PLANT CRUDE EXTRACT - (reply: 1)
  15. general question about enzyme kinetic parameters kcat specific activity... - (reply: 2)
  16. strange enzyme assay - (reply: 6)
  17. restriction enzyme sites - disruption (reply: 4)
  18. how to find the modification enzyme of a target protein - (reply: 1)
  19. In vitro enzyme assay - (reply: 1)
  20. Store restrict Enzyme in working system - (reply: 5)
  21. in vitro enzyme assay - (reply: 2)
  22. Enzyme Activity - (reply: 2)
  23. Enzyme Kinetics - (reply: 2)
  24. lux gene cloning using Sal I as restriction enzyme - (reply: 1)
  25. Release of an insert from the cloning vector using restriction enzymes - (reply: 1)
  26. phytase enzyme assay - (reply: 6)
  27. Tool for complementary restriction enzymes? - REs producing overhangs to religate (reply: 5)
  28. Restriction Enzyme Troubleshooting Help? - (reply: 2)
  29. How many bases should be added to end of enzyme in PCR for effective cutting - (reply: 8)
  30. restriction enzyme digestion problem - (reply: 8)
  31. Can a UV crosslinker be used on enzymes, reagents and liquids in general? - (reply: 2)
  32. Enzyme Unit - (reply: 5)
  33. How to store enzyme for long storage? - (reply: 2)
  34. Enzyme kinetics by Reverse Phase HPLC? - (reply: 4)
  35. Recipe for Impron II buffer (Promega) - We have excess enzyme and not enough buffer! (can't belive it myself) (reply: 2)
  36. Can enzyme kinetics be measured in whole cell extracts? - (reply: 3)
  37. Enzyme kinetics - (reply: 1)
  38. Enzymes in vitro and in vivo - Activities, temperature optima and all (reply: 3)
  39. RE Enzyme Aliquot Dates - Just from curiousity... (reply: 1)
  40. Enzyme activity - (reply: 6)
  41. Enzyme kinetic kcat, kcat/Km - (reply: 1)
  42. restriction enzyme - can i cut the noncoding region? - (reply: 2)
  43. only one band after double enzyme digestion - (reply: 4)
  44. My enzyme is not active :( - (reply: 3)
  45. Preparation of lysis buffer for enzyme extraction - (reply: 5)
  46. NEB restriction enzyme BssS1 - digest (reply: 1)
  47. using restriction enzymes for studying DNA methylation - hemimethylation (reply: 3)
  48. For double sequential digest: which enzyme first? - (reply: 7)
  49. restriction enzyme site with primers - (reply: 4)
  50. Enzymes in 10 x buffer - (reply: 2)
  51. Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
  52. Enzyme Activity - efficiency of inhibition (reply: 16)
  53. Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
  54. enzyme extraction - (reply: 3)
  55. help trouble shooting: PstI/SapI cloning - Is SapI a nasty enzyme? (reply: 6)
  56. Restricition enzyme qouted in paper doesnot cut the PCR product according to the - (reply: 3)
  57. Which enzyme for repairing nicks in ligated constructs? - (reply: 1)
  58. 4 enzyme digest and 3 piece ligation? - (reply: 3)
  59. Amt of restriction enzyme - (reply: 4)
  60. Restriction enzyme sites mutate after ligation? - (reply: 2)
  61. enzyme extraction : linamarase - questions about the cassava leaf enzyme extraction experiment. (reply: 1)
  62. Using single restriction enzyme SalI for cloning to a binary vector. How I will - Please its really very urgent...help me...help me.. (reply: 4)
  63. HELP in Restriction Enzyme Digestion - troubleshooting re digestion (reply: 3)
  64. Problems with certain restriction enzymes - on the verge of tears - (reply: 10)
  65. Enzyme sites on Primer - (reply: 3)
  66. Ligate with 01 enzyme site - (reply: 5)
  67. database , enzymes, bacteria - (reply: 1)
  68. Problem with restriction enzyme NdeI - How many bases do I need? (reply: 6)
  69. Handling standard deviations in enzyme kinetics? - Kcat/Km (reply: 3)
  70. PstI/SapI digestion problem - restriction enzyme (reply: 3)
  71. Double restriction enzyme digest - (reply: 3)
  72. enzyme activity - enzymes (reply: 1)
  73. NheI, EcoRI vector double digest - Troubling getting second enzyme to cut (reply: 6)
  74. how to switch to a gene in a vector using PCR or restriction enzymes? - gene is in pIVS2 vector (reply: 3)
  75. Restriction Enzyme Binding - (reply: 3)
  76. Problems with restriction enzyme digest. - (reply: 2)
  77. Overtreatment by dephosphorylating enzymes - (reply: 7)
  78. Double Digestion with Single Restriction Enzyme - (reply: 2)
  79. lost of enzyme activity after thawing - (reply: 3)
  80. Restriction enzyme digestion problem - (reply: 4)
  81. Concerning digestion enzymes - How do digestion enzymes work really? (reply: 1)
  82. enzyme site after blunting - (reply: 7)
  83. Why should it be digested with a restriction enzyme before treated with bisulfi - (reply: 5)
  84. Ultrafiltration of Enzyme Solution? - (reply: 4)
  85. Where exactly does a restriction enzyme cut in its recognition site? - (reply: 1)
  86. Enzyme dilution. - (reply: 8)
  87. Question about Restriction enzyme - (reply: 3)
  88. proteolytic enzymes - (reply: 1)
  89. Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
  90. Do restriction enzymes work on dUTP? - (reply: 1)
  91. Engineering Enzyme Site - (reply: 1)
  92. Overview of restriction enzyme activity... - ...depending on number of bases flanking? (reply: 2)
  93. restriction enzymes primers - (reply: 5)
  94. Kpn1 restriction enzyme - (reply: 12)
  95. EADIE-HOFSTEE enzyme kinetics plot - (reply: 1)
  96. enzyme assay - (reply: 5)
  97. ChIP- any experiences in using restriction enzymes instead of sonication? - (reply: 4)
  98. Calculating specific enzyme activity - (reply: 6)
  99. Restriction with two incompatible enzymes - (reply: 4)
  100. Enzymes - (reply: 1)
  101. Which enzyme is to be digested first in sequential RE? - In vector preparation for cloning... (reply: 7)
  102. digesting DNA with buffer incompatible enzymes - (reply: 4)
  103. Anyone used restriction enzyme BsaI? - (reply: 3)
  104. PCR using an enzyme mix - never done this before, how do I set it up? (reply: 4)
  105. Primers and enzymes not compatible - How can I fix this? (reply: 2)
  106. In Bradford assay, what factors affect the measurement of enzyme (BSA) specific - (reply: 5)
  107. Enzyme Site in reverse Primer - (reply: 3)
  108. If you did an enzyme catalysing reaction and then wanted to draw a graph....? - (reply: 2)
  109. In enzyme kinetics, how can i calculate the proportion of substrate used up? - (reply: 2)
  110. Restriction enzyme specificity for thymine and DNA - are thymine/uracil and DNA/RNA interchangeable? (reply: 1)
  111. Ultrasonic homogenizer for protein and enzyme extraction? - (reply: 3)
  112. Is restriction enzyme Srf1 a good cutter? - (reply: 4)
  113. Protocol for DNA Methylation analysis using restriction enzyme digest - (reply: 10)
  114. Restriction enzyme -Eco47III - (reply: 1)
  115. impossible single enzyme cut - (reply: 1)
  116. PCR enzyme activity-can you do 50 cycles - (reply: 2)
  117. which restriction enzyme should I use to digest the vector? - (reply: 3)
  118. Does Ammonium Sulfate Fraction of Protein and untrafiltration effect to enzyme a - (reply: 2)
  119. Using enzyme extinction curves to plot Km and Vmax - (reply: 2)
  120. Using enzyme extinction curves to plot Km and Vmax - (reply: 5)
  121. NAD+/NADH enzyme cycling assay in plants--HELP - enzyme assays (reply: 2)
  122. Restriction Enzyme and Sequencing Problems - (reply: 1)
  123. restriction enzymes - (reply: 4)
  124. enzyme inhibition by glycerol - (reply: 2)
  125. enzyme units and IU - (reply: 9)
  126. double restriction enzyme digestion - not work? (reply: 6)
  127. My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
  128. Enzyme or probe - who's the trouble maker in my southerns? - (reply: 1)
  129. Southern Blot: which restriction enzyme? - (reply: 1)
  130. endothelial lipase - concentration of the enzyme without loss of activity (reply: 5)
  131. controls for enzyme assay - (reply: 1)
  132. Long range PCR - What enzymes do you use? - (reply: 6)
  133. Glycerol for enzyme buffer - (reply: 4)
  134. Can DNA ethanol precipitation remove restriction enzyme? - (reply: 7)
  135. ENZYME RECONSTITUTION - (reply: 4)
  136. enzyme activity assay - succinate dehydrogenase (reply: 2)
  137. How to add restriction enzyme sites to the primer - (reply: 6)
  138. pGEM-T Restriction Enzymes won't cut out PCR clone - (reply: 3)
  139. Enzyme inhibition assay - Use of fluorescence microplate reader (reply: 7)
  140. Imidazole + enzyme activity - (reply: 6)
  141. exciting enzyme - (reply: 4)
  142. enzyme that removes 5' overhangs - (reply: 1)
  143. how to ligate two ends with different enzyme site - (reply: 3)
  144. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
  145. plasmid DNA and restrictions enzyme - (reply: 6)
  146. problems with NdeI restriction enzyme? - (reply: 2)
  147. Reasons for enzyme purification? - (reply: 3)
  148. help with SAl 1 enzyme.. - base sequence (reply: 6)
  149. protein/enzyme activity asssay - (reply: 1)
  150. formula for enzyme units - (reply: 1)
  151. effect of enzymes on cell membrane - (reply: 4)
  152. enzyme purification - (reply: 3)
  153. Enzyme Heat Shock - (reply: 3)
  154. What is the PAHBAH method for enzyme assay? - (reply: 1)
  155. What is the PAHBAH method for enzyme assay? - (reply: 1)
  156. How to freeze dry / lyophilize enzymes in microplate wells - (reply: 1)
  157. southern and restriction enzyme - what enzyme to choose (reply: 6)
  158. enzyme - (reply: 2)
  159. Role of BSA in restriction enzyme digestion - help (reply: 2)
  160. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
  161. Lipase enzyme digestion of milk experiment - effects of stale milk - Help needed understanding how stale milk could affect my experiment... (reply: 2)
  162. bee venom peptides, proteins, enzymes - (reply: 1)
  163. which enzyme should I select? - (reply: 2)
  164. Enzyme activity - Enzyme activity (reply: 5)
  165. Restriction enzyme that cut CGCGCG? - (reply: 3)
  166. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
  167. Can enzyme be diluted in glycerol!? - (reply: 1)
  168. proteolytic digests - which enzymes cut where.... (reply: 6)
  169. Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
  170. Enzyme units - (reply: 5)
  171. enzyme activity measurement - (reply: 1)
  172. Enzyme Restriction - (reply: 4)
  173. attach adaptors or PCR the restriction enzyme sites? - which one would you suggest and why (reply: 2)
  174. Partial digest to destroy an enzyme site - (reply: 10)
  175. restriction enzyme specificity - (reply: 7)
  176. how do you inactivate the enzymes after digestion? - (reply: 17)
  177. ACE PCR - problems with PCR for angiotensin-converting enzyme (reply: 2)
  178. why the inserted fragment can not be cutted ..... - the inserted fragment can not cutted by the enzymes used (reply: 1)
  179. How 3M KAc to influence restriction enzyme activity? - (reply: 4)
  180. enzymes - (reply: 13)
  181. method of using the CpG methylase enzyme - (reply: 7)
  182. how do you check your restriction enzyme is working? - (reply: 12)
  183. Restriction Enzyme BstBI - (reply: 2)
  184. protein/enzyme activity - lost activity after his tagging (reply: 1)
  185. digestion with pvu1 enzyme - (reply: 3)
  186. double enzyme cut and ligation - (reply: 3)
  187. problems with same enzyme cutted DNAs ligation - (reply: 1)
  188. Restriction enzyme SBFI failed to cut plasmid - (reply: 3)
  189. restriction enzyme digestion problem - (reply: 7)
  190. shearing chromatin with enzymes - (reply: 4)
  191. help! t4 kinase reaction - Does SDS inactivates the enzyme? (reply: 4)
  192. Question about activity of restriction enzymes - (reply: 3)
  193. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  194. restriction enzyme inactivation - How do you inactivate restriction enzymes? (reply: 4)
  195. restriction enzyme mapping - (reply: 1)
  196. Why a bacterial cell restriction enzyme not cleave foreign gene - (reply: 8)
  197. Problems with restriction enzymes - (reply: 4)
  198. Questio on Southern blot, enzyme to be used? - (reply: 1)
  199. Most important to know - effect of enzymes on DNA (reply: 2)
  200. Difference between MSP1, HAPA2 and SAU3A1 - restirction enzymes (reply: 1)
  201. plasmids turnedinto smears after NcoI digestion - restriction enzyme problem (reply: 1)
  202. Lyophilized enzymes - (reply: 1)
  203. enzyme dilution help - (reply: 4)
  204. Question about cloning a gene coding an enzyme - (reply: 1)
  205. small sized restricted bands couldnt be visualized - restriction enzymes problems (reply: 22)
  206. Most Peculiar Problem with an enzyme activity - 4 -years of nothing (reply: 2)
  207. Enzyme digestion before bisulfite treatment - (reply: 1)
  208. methylation enzymes - (reply: 1)
  209. Do enzymes work in different buffers? - (reply: 2)
  210. CViJI restriction enzyme? - (reply: 2)
  211. Restriction enzymes cut reaction - (reply: 1)
  212. plasmid digest - restriction enzyme won´t cut (reply: 1)
  213. Digestion time and enzyme - (reply: 2)
  214. Frequency of cutting of recognition enzymes - (reply: 3)
  215. Invitrogen platinum pfx enzyme not so fantastic - problems with pcr product gel migration (reply: 1)
  216. Help! Who has surplus enzymes - Nuclease I? - (reply: 1)
  217. How to identify plasmid - using enzyme and PCR or other? (reply: 2)
  218. enzyme activity - (reply: 2)
  219. enzyme markers - use of specific enzymes to measure fractionation success (reply: 1)
  220. Preservation of enzyme - (reply: 1)
  221. plasmid can't be digested by enzyme - (reply: 13)
  222. enzyme activity - (reply: 1)
  223. Mutational Event, Wild type enzyme Sequence - (reply: 2)