Restriction enzyme -Eco47III - (Oct/24/2006 )

Hi all,

Does anyone have experience working with the enzyme Eco47III?
What is the efficiency of digestion? Are there any precautions one should take while using it?

Thanks...

CD

well the digestion is relative simple, but in case of vectors you need to dephosphorylate efficiently.
I have better results by [adding 1µl CIP then 15' 37° 15' 56°] twice