Enzymes in vitro and in vivo - Activities, temperature optima and all (Mar/25/2008 )
Dear all,
I have been thinking about this for a while and decided to discuss with you guys here. As we know, most cellular reactions (in vivo) can now be done in vitro. I believe both in vivo and in vitro are somewhat different (as far as enzymes are concerned).
Reason: In the in vivo environment, there is better regulation due to the interconnected nature of some cycles. A positive/negative feedback exists. Also, suppressors exists in cells but not in vitro thus making reactions in both condition different.
I'm not too sure of my explanations and hope for more feedback here. For the same enzyme, does the natural ones and purified ones behave identically in vivo and in vitro?
Thanks.
I want to add question... What procedure is made to ensure the optimum reaction? Let say I have an enzyme,a 1st subtrate and second subtrate... what should I do?
I have been thinking about this for a while and decided to discuss with you guys here. As we know, most cellular reactions (in vivo) can now be done in vitro. I believe both in vivo and in vitro are somewhat different (as far as enzymes are concerned).
Reason: In the in vivo environment, there is better regulation due to the interconnected nature of some cycles. A positive/negative feedback exists. Also, suppressors exists in cells but not in vitro thus making reactions in both condition different.
I'm not too sure of my explanations and hope for more feedback here. For the same enzyme, does the natural ones and purified ones behave identically in vivo and in vitro?
Thanks.
I have been thinking about this for a while and decided to discuss with you guys here. As we know, most cellular reactions (in vivo) can now be done in vitro. I believe both in vivo and in vitro are somewhat different (as far as enzymes are concerned).
Reason: In the in vivo environment, there is better regulation due to the interconnected nature of some cycles. A positive/negative feedback exists. Also, suppressors exists in cells but not in vitro thus making reactions in both condition different.
I'm not too sure of my explanations and hope for more feedback here. For the same enzyme, does the natural ones and purified ones behave identically in vivo and in vitro?
Thanks.
you are right; in cellulo, enzymes are integrated in a network of activator and deactivators, and normally do not behave as in state of purity and in vitro; to simulate in vivo conditions in vitro is difficult as the network is complex
[quote name='kent19' date='Mar 25 2008, 10:21 PM' post='130350']
I want to add question... What procedure is made to ensure the optimum reaction? Let say I have an enzyme,a 1st subtrate and second subtrate... what should I do?
do you think in terms of Michaelis-Menten kinetics? you have to offer increasing amounts of each substrate and to measure each specific enzyme activity; Km is a t have maximum specific activity (velocity of reaction)