How 3M KAc to influence restriction enzyme activity? - (Nov/01/2005 )
I precipitate my PCR production (~200bp) with 3M KAc and absolute alcohol ,and spin down it, then wash it by 75%alcohol , the production will be digested by enzymes, i don't know whether the 3M KAc will be removed by 75%alcohol .if it remain in my DNA solution,whether it will inactivate my enzyme.any suggestion will be appreciated.waiting............. 
Yes, the purpose of the 75% alcohol (I use 70%) is to remove the salt. If you are concerned about salt concentration, then you can wash the pellet a second time, or use a larger volume of alcohol.
(twice is more effective). There is a lot of salt in most restriction buffers anyway, so I would not be too concerned.
(twice is more effective). There is a lot of salt in most restriction buffers anyway, so I would not be too concerned.
thanks for your answer,and i wanna know what about the Ac-?
pfy1982
sodium acetate is a salt, as is potassium acetate, or whatever acetate you are adding...acetate is the salt component of acetic acid, yes?
[quote name='aimikins' date='Nov 4 2005, 09:35 AM' post='29985']
pfy1982
sodium acetate is a salt, as is potassium acetate, or whatever acetate you are adding...acetate is the salt component of acetic acid, yes?
[/q
yup! thx for your oppugn.