Restriction enzymes cut reaction - (Nov/16/2004 )

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Hi! I'm wonderful If anyone has answer as to "Why I don't cut plasmid pET22b(+) by BamHI and XhoI. When I cut it with normal protocol then I don't ligate with 500 bp fragment"

I have also problems with the pET22b vector... We found out that it is possible to cut by sequentiell digestion but not by double digest. Perhaps this will help you...