Enzyme dilution. - (May/03/2007 )

Hello, I have two very stupid questions-I have an enyzme package of 50 UN in 10 mikroliters and this is 7.690 units/ml. These 7.690 units/ml-is that mean seven thousand six hundred and so on? And how can I prepare solution of 5 UN/ml?

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i think it's 7 units and something ....it's written as 'point' not 'comma'
and for preparing 5 units, you have to dilute

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Please clarify your question; how much is the concentration of your stock solution?

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My stock solution is 50 UN in 10 mikroliters. I asked the company and they wrote me that package of 50 UN contains 7.690 units/ml. What I need is 5 UN/ml. I am not familiar with enzymes at all, could you give me please any clues?

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Is'nt UN the abbreviation of unit?

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you have to check how the company defines "unit" as it is not a term of SI;

what one company provides as "U" is defined as "mU" for another company;

never heard of UN; UN is neither unit nor unit/vol from your description...

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i tried to search for " UN", but i found nothing about it

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I don't know about what UN stands for but I would also guess it meant units, but I would check with the company.
In regard to the 7,690- I agree with strawberry, its 7 units and something, many countries use a comma as a decimal place

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Thanks everybody
The enzyme is product of Sigma, I am reading the technical bulletin.......What is written there is "One unit will convert 1 nanomole substratum in 1 minute at 37 deg., at pH 7.5. One Sigma unit is equal to 1 IUB milliunit." Well, I feel even more confused

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