enzyme markers - use of specific enzymes to measure fractionation success (Apr/06/2002 )
I am using centrifugation to fractionate cytosolic and mitochondrial fractions. I would like to know if there is a cytosolic (and a mitochondrial)-exclusive enzyme that can be assayed in order to make sure that the correct fraction has been obtained
-grant-
cytosolic markers Phosphofructokinase, or hexokinase, or even aldolase..for mitochondrial preps...cytochrome C, ornithine transcarbamoylase, pyruvate dehydrogenase
-alchemist-