EADIE-HOFSTEE enzyme kinetics plot - (Feb/28/2007 )
I am just not understanding how these things work.
Using this plot as an example (attached).
Can someone explain how one would go about generating this. I mean obviously you dont have the raw data to generate it, but in theory? Thanks.
-grahamkeith-
QUOTE (grahamkeith @ Mar 1 2007, 12:49 AM)
I am just not understanding how these things work.
Using this plot as an example (attached).
Can someone explain how one would go about generating this. I mean obviously you dont have the raw data to generate it, but in theory? Thanks.
Using this plot as an example (attached).
Can someone explain how one would go about generating this. I mean obviously you dont have the raw data to generate it, but in theory? Thanks.
v (activity) is a function of v/[substrate]; you measure v at different conc of substrate and plot as shown; slope corresponds with Km; linearity tells you sth about cooperativity
-The Bearer-