|
31. qPCR plate setup - too many samples for one plate, how to divide samples? (reply: 1)
32. qPCR troubles - (reply: 5)
33. CT value of reerence gene - what should be the GAPDH Ct value (reply: 1)
34. qPCR 96 well plate - (reply: 2)
35. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
36. confused with plots - Rn vs. cycle & delta Rn vs. cycle - (reply: 1)
37. Individual or Combined samples - Run each sample separately-or combine and run once (reply: 1)
38. transgene copy number determination with Q-PCR - (reply: 1)
39. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
40. Why not just two points on a standard curve? - (reply: 1)
41. Calculation issue for inconsistent results in triplicates in Taqman rPCR. - (reply: 10)
42. Compression pad? - (reply: 2)
43. PCR = products come randomly? - (reply: 4)
44. pcr question - (reply: 5)
45. Redundancy of real time PCR results - (reply: 2)
46. RNA extraction for real time PCR from specific brain region - (reply: 1)
47. Normal PCR for looking expression - (reply: 1)
48. Best chemistry for relative quantitation of gene expression - Give your opinion on the best chemistry according to your experience (reply: 5)
49. qRT-PCR primer design - (reply: 1)
50. Real Time PCR Efficiency - (reply: 3)
51. genomic DNA contamination in RT-PCR - (reply: 7)
52. gene expression analysis - (reply: 1)
53. Problems with second use of diluted cDNA in qPCR - (reply: 4)
54. comparative quantitation - (reply: 1)
55. Calibrator undetectable - (reply: 1)
56. qRT-PCR - data analysis - methods that DO NOT use a calibrator ! (reply: 3)
57. housekeeping genes for brain tissue - (reply: 4)
58. First strand cDNA synthesis for qRT-PCR - (reply: 1)
59. qRT-PCR control - (reply: 1)
60. exogenous control - (reply: 2)
32. qPCR troubles - (reply: 5)
33. CT value of reerence gene - what should be the GAPDH Ct value (reply: 1)
34. qPCR 96 well plate - (reply: 2)
35. What grade of primers for real-time TaqMan PCR assays? - (reply: 1)
36. confused with plots - Rn vs. cycle & delta Rn vs. cycle - (reply: 1)
37. Individual or Combined samples - Run each sample separately-or combine and run once (reply: 1)
38. transgene copy number determination with Q-PCR - (reply: 1)
39. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
40. Why not just two points on a standard curve? - (reply: 1)
41. Calculation issue for inconsistent results in triplicates in Taqman rPCR. - (reply: 10)
42. Compression pad? - (reply: 2)
43. PCR = products come randomly? - (reply: 4)
44. pcr question - (reply: 5)
45. Redundancy of real time PCR results - (reply: 2)
46. RNA extraction for real time PCR from specific brain region - (reply: 1)
47. Normal PCR for looking expression - (reply: 1)
48. Best chemistry for relative quantitation of gene expression - Give your opinion on the best chemistry according to your experience (reply: 5)
49. qRT-PCR primer design - (reply: 1)
50. Real Time PCR Efficiency - (reply: 3)
51. genomic DNA contamination in RT-PCR - (reply: 7)
52. gene expression analysis - (reply: 1)
53. Problems with second use of diluted cDNA in qPCR - (reply: 4)
54. comparative quantitation - (reply: 1)
55. Calibrator undetectable - (reply: 1)
56. qRT-PCR - data analysis - methods that DO NOT use a calibrator ! (reply: 3)
57. housekeeping genes for brain tissue - (reply: 4)
58. First strand cDNA synthesis for qRT-PCR - (reply: 1)
59. qRT-PCR control - (reply: 1)
60. exogenous control - (reply: 2)