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361. Two Tm peak in real-time but only one product on a gel? - What's goning on????? (reply: 4)
362. PCR efficiency - (reply: 2)
363. real time PCR using two sets of primers with different melt curves - (reply: 4)
364. How big a problem is cDNA sticking to the plastic? - (reply: 3)
365. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
366. Real-time PCR optimization - (reply: 8)
367. Degenerated qPCR data after keeping the cDNA in the fridge. - (reply: 2)
368. how many cycles needed? - (reply: 4)
369. Real-time PCR....Making sense of it all? - (reply: 3)
370. Real-time PCR vs. Northern Blotting / mRNA stability assays... - Advice from those who have experience with mRNA stability assays, plea (reply: 2)
371. Testing newly designed primers prior to real time - (reply: 2)
372. Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
373. impossible gene ! - (reply: 3)
374. How long can the dilution series of standard be store in 4oC? - (reply: 2)
375. high efficiency and what to do with that? - (reply: 7)
376. Efficiency of DIG Labeling kit (Roche) - (reply: 1)
377. initial amount of cDNA - (reply: 2)
378. Baseline/threshold adjustment and efficiencies - (reply: 1)
379. 18S rRNA as internal control for mRNA-cDNA? - internal control (reply: 1)
380. Comparison across multiple plates - (reply: 3)
381. Real-time PCR to analyze DNA methylation - (reply: 3)
382. Primer concentration and standard curve slope in "Relative Standard Curve M - (reply: 1)
383. Bio-Rad experion - is there a manual for this (reply: 3)
384. problem with -ve controls of mt genes - (reply: 1)
385. Dissociation curve vs Gel image - Which one to trust? (reply: 1)
386. High Ct Values - (reply: 1)
387. confirming equal expression of transcripts in three samples...how to? - (reply: 3)
388. Traget gene has the same Ct as HK gene - what to do ? - HELP !!!!!!!!! (reply: 4)
389. optimising - ct versus efficiency - (reply: 1)
390. Cell count vs Reference gene - Cell count vs Reference gene (reply: 1)
362. PCR efficiency - (reply: 2)
363. real time PCR using two sets of primers with different melt curves - (reply: 4)
364. How big a problem is cDNA sticking to the plastic? - (reply: 3)
365. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
366. Real-time PCR optimization - (reply: 8)
367. Degenerated qPCR data after keeping the cDNA in the fridge. - (reply: 2)
368. how many cycles needed? - (reply: 4)
369. Real-time PCR....Making sense of it all? - (reply: 3)
370. Real-time PCR vs. Northern Blotting / mRNA stability assays... - Advice from those who have experience with mRNA stability assays, plea (reply: 2)
371. Testing newly designed primers prior to real time - (reply: 2)
372. Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
373. impossible gene ! - (reply: 3)
374. How long can the dilution series of standard be store in 4oC? - (reply: 2)
375. high efficiency and what to do with that? - (reply: 7)
376. Efficiency of DIG Labeling kit (Roche) - (reply: 1)
377. initial amount of cDNA - (reply: 2)
378. Baseline/threshold adjustment and efficiencies - (reply: 1)
379. 18S rRNA as internal control for mRNA-cDNA? - internal control (reply: 1)
380. Comparison across multiple plates - (reply: 3)
381. Real-time PCR to analyze DNA methylation - (reply: 3)
382. Primer concentration and standard curve slope in "Relative Standard Curve M - (reply: 1)
383. Bio-Rad experion - is there a manual for this (reply: 3)
384. problem with -ve controls of mt genes - (reply: 1)
385. Dissociation curve vs Gel image - Which one to trust? (reply: 1)
386. High Ct Values - (reply: 1)
387. confirming equal expression of transcripts in three samples...how to? - (reply: 3)
388. Traget gene has the same Ct as HK gene - what to do ? - HELP !!!!!!!!! (reply: 4)
389. optimising - ct versus efficiency - (reply: 1)
390. Cell count vs Reference gene - Cell count vs Reference gene (reply: 1)